用于高灵敏度表面增强拉曼散射检测同型半胱氨酸的银纳米线/金纳米球二元等离子体组装膜
Silver nanowire/gold nanosphere binary plasma-assembled membranes for sensitive SERS detection of homocysteine.
作者信息
Peng Shirun, Zheng Yujun, Li Wanjing, Lin Minghuan, Wu Zhihong, You Ruiyun, Lin Qingqiang, Wu Yaling
机构信息
School of Materials and Chemical Engineering, Fuzhou Institute of Oceanography, Minjiang University, Fuzhou, Fujian, 350108, China.
College of Chemistry and Materials Science, Fujian Provincial Key Laboratory of Advanced Oriented Chemical Engineer, Fujian Key Laboratory of Polymer Materials, Engineering Research Center of Industrial Biocatalysis, Fujian Normal University, Fujian Province Higher Education Institutes, Fuzhou, Fujian, 350007, China.
出版信息
Mikrochim Acta. 2024 Dec 26;192(1):32. doi: 10.1007/s00604-024-06891-3.
Silver nanowire (Ag NW)/gold nanosphere (Au NS) binary plasma films were prepared using plasma coupling between Ag NWs and Au NSs. The plasma films formed by combining these two noble metals showed better sensitivity for SERS detection with a minimum detection concentration of 10 M for R6G compared to pure Ag NWs or Au NSs. After rational optimisation of the substrate preparation process, the substrate showed good homogeneity, reproducibility and stability. We perform an indirect detection of homocysteine (Hcy) through a specific reaction of Hcy with o-phthalaldehyde (OPA). The lowest detectable concentration of Hcy was 5 × 10 M. The recoveries of Hcy were 94.53 ~ 103.43% with the relative standard deviations (RSDs) of 2.53%, 9.21% and 12.26%, respectively, in the spike recovery experiments. With good selectivity and accuracy for Hcy detection, this plasma film provides an idea for the detection of disease markers in serum.
通过银纳米线（Ag NW）与金纳米球（Au NS）之间的等离子体耦合制备了银纳米线/金纳米球二元等离子体薄膜。与纯银纳米线或金纳米球相比，由这两种贵金属组合形成的等离子体薄膜对表面增强拉曼光谱（SERS）检测表现出更好的灵敏度，对罗丹明6G（R6G）的最低检测浓度为10⁻⁶ M。在对基底制备工艺进行合理优化后，该基底表现出良好的均匀性、重现性和稳定性。我们通过同型半胱氨酸（Hcy）与邻苯二甲醛（OPA）的特异性反应对Hcy进行间接检测。Hcy的最低可检测浓度为5×10⁻⁸ M。在加标回收实验中，Hcy的回收率为94.53%~103.43%，相对标准偏差（RSD）分别为2.53%、9.21%和12.26%。这种等离子体薄膜对Hcy检测具有良好的选择性和准确性，为血清中疾病标志物的检测提供了一种思路。
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