Huang Jiaqi, Wang Mengyao, Wang Yu, Sun He, Zou Yadan, Tian Xiaojin, Cui Dianxin, Zhang Peng, Li Xue, Yang Wenzhi
State Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Tianjin 301617, China; Haihe Laboratory of Modern Chinese Medicine, 10 Poyanghu Road, Tianjin 301617, China.
State Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Tianjin 301617, China; Haihe Laboratory of Modern Chinese Medicine, 10 Poyanghu Road, Tianjin 301617, China.
Fitoterapia. 2025 Mar;181:106371. doi: 10.1016/j.fitote.2024.106371. Epub 2024 Dec 24.
Prunella vulgaris is a medicinal and edible homologous plant, commonly used as a folk medicine to treat diseases. The Prunella vulgaris polysaccharides (PVPs) are reported with the antioxidant activity. This work was designed to isolate, characterize, and test the antioxidant activity of purified PVPs from P. vulgaris. A new homogeneous polysaccharide (PVP-1) was prepared by the DEAE column from PVPs, and diverse chromatography/spectroscopy and chemical methods were simultaneously employed to characterize the fine structure of PVP-1. The results showed PVP-1 had a triple helix structure, and the repeating structural unit of PVP-1 was composed of →6)-β-D-Galp-(1→6)-β-D-Galp-(3,1→6)-β-D-Galp-(1→6)-β-D-Galp-(1→ as the main chain, together with →6)-β-D-Galp-(1,3→1)-α-D-Araf-(5→1)-β-D-Galp-(4→1)-α-D-Galp-(2→ and →6)-β-D-Galp-(1,3→1)-α-D-GlcAp-(4→1)-α-D-Glcp-(4→1)-α-D-Galp as the branch chains. The main monosaccharides of PVP-1 were galactose (Gal, 41.25 %), galactose-OMe (Gal-OMe, 27.73 %), arabinose (Ara, 10.63 %), mannose (Man, 9.86 %), glucose (Glc, 3.88 %), glucuronic acid (GlcA, 2.86 %), ribose (Rib, 1.79 %), and xylose (Xyl, 1.76 %). In addition, the scanning electron microscopy (SEM) displayed that the surface of PVP-1 was rough and porous. PVP-1 gave the scavenging rates of the DPPH, ABTS, and hydroxyl radical lower than vitamin C at the same concentration, with the highest scavenging rate of DPPH radical at 82.71 % ± 4.19 % (5 mg/mL).
夏枯草是一种药食同源植物，常用作治疗疾病的民间药物。据报道，夏枯草多糖（PVPs）具有抗氧化活性。本研究旨在从夏枯草中分离、表征和测试纯化的PVPs的抗氧化活性。通过DEAE柱从PVPs中制备了一种新的均一多糖（PVP-1），并同时采用多种色谱/光谱和化学方法表征PVP-1的精细结构。结果表明，PVP-1具有三螺旋结构，其重复结构单元以→6)-β-D-吡喃半乳糖-(1→6)-β-D-吡喃半乳糖-(3,1→6)-β-D-吡喃半乳糖-(1→6)-β-D-吡喃半乳糖-(1→为主链，同时以→6)-β-D-吡喃半乳糖-(1,3→1)-α-D-阿拉伯呋喃糖-(5→1)-β-D-吡喃半乳糖-(4→1)-α-D-吡喃半乳糖-(2→和→6)-β-D-吡喃半乳糖-(1,3→1)-α-D-葡萄糖醛酸-(4→1)-α-D-葡萄糖-(4→1)-α-D-吡喃半乳糖为支链。PVP-1的主要单糖为半乳糖（Gal，41.25%）、半乳糖甲醚（Gal-OMe，27.73%）、阿拉伯糖（Ara，10.63%）、甘露糖（Man，9.86%）、葡萄糖（Glc，3.88%）、葡萄糖醛酸（GlcA，2.86%）、核糖（Rib，1.79%）和木糖（Xyl，1.76%）。此外，扫描电子显微镜（SEM）显示PVP-1的表面粗糙且多孔。在相同浓度下，PVP-1对DPPH、ABTS和羟基自由基的清除率低于维生素C，其中对DPPH自由基的清除率最高，为82.71%±4.19%（5mg/mL）。
