Liu Dong, He Yaling, Zhang Xinmiao, Ye Ziyang, Ye Ming
Engineering Research Center of Bio-process, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China; Anqing Vocational and Technical College, Anqing 246003, China.
Engineering Research Center of Bio-process, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China.
Fitoterapia. 2025 Mar;181:106390. doi: 10.1016/j.fitote.2025.106390. Epub 2025 Jan 11.
LEP-1a, a new polysaccharide fraction isolated and purified from Lachnum YM40, has a molecular weight of 24.29 kDa. LEP-1a's chemical composition investigation showed that mannose, galactose, and glucose made up the majority of its cosmetics. The methylation, nuclear magnetic resonance, and Fourier transform infrared investigations demonstrated that the (1 → 2)-β-D-Galp, (1 → 2,6)-α-D-Manp, glycosidic connections of LEP-1a were comprised of 1→)-α-D-Manp, (1 → 4)-α-D-Galp, (1 → 6)-α-D-Manp, (1 → 2)-β-D-Glcp, (1 → 4)-β-D-Glcp, (1 → 2)-α-D-Manp, and (1 → 3, 6)-β-D-Manp. LEP-1a has a linear microscopic morphology, as demonstrated by atomic force microscopy, scanning electron microscopy, and rheological property investigation. Moreover, the polysaccharide LEP-1a displayed bile acid- and cholesterol-binding capacities and inhibitory activity on lipase.
LEP - 1a是从拉氏菌YM40中分离纯化得到的一种新的多糖组分，分子量为24.29 kDa。对LEP - 1a的化学成分研究表明，甘露糖、半乳糖和葡萄糖占其组成的大部分。甲基化、核磁共振和傅里叶变换红外光谱研究表明，LEP - 1a的糖苷键由(1→2)-β - D - Galp、(1→2,6)-α - D - Manp、(1→)-α - D - Manp、(1→4)-α - D - Galp、(1→6)-α - D - Manp、(1→2)-β - D - Glcp、(1→4)-β - D - Glcp、(1→2)-α - D - Manp和(1→3,6)-β - D - Manp组成。原子力显微镜、扫描电子显微镜和流变学性质研究表明，LEP - 1a具有线性微观形态。此外，多糖LEP - 1a表现出胆汁酸和胆固醇结合能力以及对脂肪酶的抑制活性。
