Song Mengke, Wang Kaidi, Xie Yucheng, Wen Xuemei, Tu Yanli, Teng Tingting, Luo Chunling, Zhang Dayi
College of Natural Resources and Environment, South China Agricultural University, Guangzhou, 510642, PR China.
Key Laboratory of Groundwater Resources and Environment Ministry of Education, Jilin University, Changchun, 130021, PR China; College of New Energy and Environment, Jilin University, Changchun, 130021, PR China.
Environ Pollut. 2025 Feb 15;367:125582. doi: 10.1016/j.envpol.2024.125582. Epub 2024 Dec 24.
Biological soil crusts (BSCs) are the main landscape on the Qinghai-Tibetan Plateau and an ecological indicator of human disturbance. Information about antibiotic resistomes in BSCs on the Qinghai-Tibetan Plateau can provide baseline for the risk assessment and management of resistomes and yet to be explored. This work investigated the profiles and geographic patterns of antibiotic resistomes in BSCs along the Lhasa River and their response to anthropogenic activities for the first time. Various antibiotic resistance genes (ARGs) were widely distributed in BSCs, but had relatively lower detection frequency and abundance comparing to soils from human disturbed sites. ARGs profiles in BSCs were separated by altitude from 3860 to 3880 m, possibly attributing to the difference in anthropogenic activities. Above 3860 m, resistomes exhibited lower abundance including total ARGs, aadA, blaSFO and tnpA-04 owing to the rare human activities; at human disturbed sites with altitude <3860 m, the detection frequency and relative abundance of tetG02, oprJ, qacEdelta1-01, and ARGs with the mechanism of efflux pump were higher and viewed as potential indicators of human activities. Anthropogenic activities potentially promoted the horizontal gene transfer of ARGs in BSCs at human disturbed sites from co-occurrence network analysis. Our findings provided fundamental information of antibiotic resistomes in BSCs on the Qinghai-Tibetan Plateau, and unraveled possible mechanisms of human disturbance in shaping antibiotic resistomes.
生物土壤结皮是青藏高原的主要地貌景观，也是人类干扰的生态指标。青藏高原生物土壤结皮中抗生素抗性组的信息可为抗性组的风险评估和管理提供基线，但尚未得到探索。这项工作首次调查了拉萨河沿线生物土壤结皮中抗生素抗性组的概况、地理格局及其对人为活动的响应。各种抗生素抗性基因（ARGs）在生物土壤结皮中广泛分布，但与人类干扰地区的土壤相比，其检测频率和丰度相对较低。海拔3860至3880米处的生物土壤结皮中的ARGs谱因海拔而不同，这可能归因于人为活动的差异。在海拔3860米以上，由于人类活动稀少，抗性组包括总ARGs、aadA、blaSFO和tnpA-04的丰度较低；在海拔<3860米的人类干扰地区，tetG02、oprJ、qacEdelta1-01以及具有外排泵机制的ARGs的检测频率和相对丰度较高，被视为人类活动的潜在指标。从共现网络分析来看，人为活动可能促进了人类干扰地区生物土壤结皮中ARGs的水平基因转移。我们的研究结果提供了青藏高原生物土壤结皮中抗生素抗性组的基础信息，并揭示了人类干扰对抗生素抗性组形成的可能机制。
