Sarma Pallavi, Barmon Debabrata, Rai Avdhesh Kumar, Kataki Amal Chandra, Sarma Anupam, Baruah Upasana, Kakoti Lopamudra, Barman Debanjana, Sharma Ratnadeep
DBT Centre for Molecular Biology and Cancer Research, Dr B. Borooah Cancer Institute, Guwahati, Assam, India.
Department of Gynaecologic Oncology, Dr B. Borooah Cancer Institute, Guwahati, Assam, India.
Cancer Rep (Hoboken). 2024 Dec;7(12):e70097. doi: 10.1002/cnr2.70097.
Screening of rural women of Assam by careHPV test for high-risk HPV (hr-HPV) DNA and Papanicolaou (PAP) test for abnormal cytology.
This prospective cross-sectional study included 480 non-pregnant women participants aged 20-70 years from Kamrup District, Assam. Two cervical scrap samples were obtained from eligible enrolled women. The Hr-HPV DNA test by CareHPV was performed with one cervical scrap, and a second cervical scrap sample was used for the Papanicolaou (PAP) test. The statistical analysis was done using RStudio for variables. A p-value < 0.05 was considered to be statistically significant.
Women having positive hr-HPV DNA outcomes were 3.33% (16/480) and 7.7% (37/480) women had positive PAP. Tobacco chewing was significantly associated with positive hr-HPV DNA (p = 0.04) and positive PAP (p = 0.03) status. Alcohol-consuming women have a significantly higher risk of positive hr-HPV DNA (p < 0.00001) and positive PAP (p-0.04) outcomes. Irregular menstruation (p = 0.004) and urogenital tract infection (p = 0.008) also have significant risk for a positive hr-HPV DNA status. The positive hr-HPV DNA status was also significant in women having > 3 numbers of children birth (p = 0.003).
We found that the positive hr-HPV DNA status among rural women in Kamrup, Assam, was significantly associated with alcohol consumption, tobacco chewing, irregular menstruation, urogenital tract infection, and more than three children birth. The abnormal cytology outcome was also substantially associated with tobacco chewing and alcohol consumption.
通过careHPV检测对印度阿萨姆邦农村女性进行高危型人乳头瘤病毒（hr-HPV）DNA筛查，并通过巴氏涂片（PAP）检测筛查异常细胞学情况。
这项前瞻性横断面研究纳入了来自阿萨姆邦卡姆鲁普地区480名年龄在20至70岁之间的非孕女性参与者。从符合条件的入选女性中获取两份宫颈刮片样本。使用一份宫颈刮片进行CareHPV的hr-HPV DNA检测，另一份宫颈刮片样本用于巴氏涂片（PAP）检测。使用RStudio对变量进行统计分析。p值<0.05被认为具有统计学意义。
hr-HPV DNA检测结果呈阳性的女性占3.33%（16/480），巴氏涂片（PAP）检测结果呈阳性的女性占7.7%（37/480）。嚼烟草与hr-HPV DNA阳性（p = 0.04）和巴氏涂片（PAP）阳性（p = 0.03）状态显著相关。饮酒女性的hr-HPV DNA阳性（p < 0.00001）和巴氏涂片（PAP）阳性（p = 0.04）结果的风险显著更高。月经不调（p = 0.004）和泌尿生殖道感染（p = 0.008）也与hr-HPV DNA阳性状态有显著风险关联。生育子女数>3个的女性中，hr-HPV DNA阳性状态也具有显著性（p = 0.003）。
我们发现，印度阿萨姆邦卡姆鲁普农村女性中hr-HPV DNA阳性状态与饮酒、嚼烟草、月经不调、泌尿生殖道感染以及生育三个以上子女显著相关。异常细胞学结果也与嚼烟草和饮酒密切相关。
