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通过定量荧光原位杂交检测人类端粒长度:被忽视的重要性及应用

Human telomere length detected by quantitative fluorescent in situ hybridization: overlooked importance and application.

作者信息

Li Xinling, Hu Dongsheng, Zhang Ming, Wang Wei

机构信息

Department of Biostatistics and Epidemiology, School of Public Health, Shenzhen University Medical School, Shenzhen, Guangdong, China.

Department of Occupational Health and Occupational Diseases, College of Public Health, Zhengzhou University, Zhengzhou, Henan, China.

出版信息

Crit Rev Clin Lab Sci. 2025 Mar;62(2):135-147. doi: 10.1080/10408363.2024.2441733. Epub 2024 Dec 26.

Abstract

The technique of Quantitative Fluorescence Hybridization (Q-FISH) plays a crucial role in determining the length of telomeres for studies in molecular biology and cytogenetics. Throughout the years, the use of Q-FISH for measuring telomere length has made substantial contributions to research in aging, cancer, and stem cells. The objective of this analysis is to delineate the categorization, fundamental concepts, pros and cons, and safety measures of Q-FISH in telomere length analysis, encapsulate, and anticipate its principal uses across diverse human biomedical research fields.

摘要

定量荧光杂交(Q-FISH)技术在分子生物学和细胞遗传学研究中确定端粒长度方面发挥着关键作用。多年来,使用Q-FISH测量端粒长度为衰老、癌症和干细胞研究做出了重大贡献。本分析的目的是阐述Q-FISH在端粒长度分析中的分类、基本概念、优缺点和安全措施,概括并预测其在不同人类生物医学研究领域的主要用途。

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