Hamamouche Kahina, Elhadj Zoubida, Khattabi Latifa, Zahnit Wafa, Djemoui Brahim, Kharoubi Omar, Boussebaa Walid, Bouderballa Mouhamed, El Moustapha Kallouche Mohammed, Attia Sabry M, Ahmad Sheikh F, Atanassova Maria, Messaoudi Mohammed
Environmental Monitoring Network Laboratory, Department of Biology, Faculty of Natural and Life Sciences, Ahmed Ben Bella University, Oran 31000, Algeria.
Laboratory for Environmental and Materials Sciences Studies, Ahmed Ben Bella University, Oran 31000, Algeria.
Mar Drugs. 2024 Nov 25;22(12):530. doi: 10.3390/md22120530.
This study represents the first investigation into the ultrasonic and microwave extraction of bioactive metabolites from (red seaweed) and () (brown seaweed), with a focus on their biological activities. The research compares ultrasound-assisted extraction (UAE) with microwave-assisted extraction (MAE) utilizing a hydromethanolic solvent to evaluate their effects on these seaweeds' bioactive compounds and biological activities. The assessment included a series of antioxidant essays: DPPH, ABTS, phenanthroline, and total antioxidant capacity, followed by enzyme inhibition activities: alpha-amylase and urease. Results revealed significant proportions of phenolic compounds, ranging from 48.31 ± 0.32 to 74.42 ± 0.80 μg GAE/mg, depending on the extraction method. The extracts demonstrated a high antioxidant activity, with IC values ranging from 26.58 ± 0.39 to 87.55 ± 0.69 μg/mL. Notably, the MAE extract of showed a value of 48.11 ± 2.75 μg/mL for alpha-amylase inhibition, which is strictly superior to the reference acarbose with an IC equal to 3431.01 μg/mL. UPLC-ESI-MS/MS analysis identified 14 bioactive compounds. The proportion of riboflavin with MAE was 70.58% and 59.11% for and fractions, respectively. These findings underscore the critical influence of extraction technique selection on bioactive compounds' yield and efficiency, highlighting the potential of algal biomass as a sustainable alternative in various applications.
本研究首次对从（红藻）和（褐藻）中超声和微波提取生物活性代谢物进行了调查，重点关注其生物活性。该研究比较了利用甲醇水溶液溶剂的超声辅助提取（UAE）和微波辅助提取（MAE），以评估它们对这些海藻生物活性化合物和生物活性的影响。评估包括一系列抗氧化试验：DPPH、ABTS、邻菲罗啉和总抗氧化能力，随后是酶抑制活性：α-淀粉酶和脲酶。结果显示，取决于提取方法，酚类化合物的比例显著，范围为48.31±0.32至74.42±0.80μg GAE/mg。提取物表现出高抗氧化活性，IC值范围为26.58±0.39至87.55±0.69μg/mL。值得注意的是，的MAE提取物对α-淀粉酶抑制的IC值为48.11±2.75μg/mL，严格优于参考阿卡波糖，其IC等于3431.01μg/mL。UPLC-ESI-MS/MS分析鉴定出14种生物活性化合物。MAE条件下核黄素的比例在和馏分中分别为70.58%和59.11%。这些发现强调了提取技术选择对生物活性化合物产量和效率的关键影响，突出了藻类生物质在各种应用中作为可持续替代品的潜力。
