优化超声与微波辅助联合萃取以提高从四种药用植物（牛至、迷迭香、金丝桃和洋甘菊）中提取生物活性化合物的效率

Optimization of Combined Ultrasound and Microwave-Assisted Extraction for Enhanced Bioactive Compounds Recovery from Four Medicinal Plants: Oregano, Rosemary, Hypericum, and Chamomile.

作者信息

Laina Konstantina Theodora, Drosou Christina, Stergiopoulos Chrysanthos, Eleni Panagiota Maria, Krokida Magdalini

机构信息

Laboratory of Process Analysis and Design, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechneiou St. Zografou Campus, 15780 Athens, Greece.

出版信息

Molecules. 2024 Dec 6;29(23):5773. doi: 10.3390/molecules29235773.

DOI:10.3390/molecules29235773

PMID:39683930

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11643802/

Abstract

This study presents the synergistic application of ultrasound- and microwave-assisted extraction (UAE-MAE) as a novel and efficient method for recovering bioactive compounds from the medicinal plants oregano, rosemary, , and chamomile. Extraction parameters, including microwave (MW) power, ultrasound (US) power, and extraction time, were optimized using the response surface methodology (RSM), with ethanol as the solvent. Extracts were evaluated for total phenolic content (TPC) via the Folin-Ciocalteu method and antioxidant activity (IC50) using the DPPH assay. High-performance liquid chromatography with diode array detection (HPLC-DAD) identified the main bioactive compounds contributing to their antioxidant and therapeutic potential. The optimized UAE-MAE conditions enhanced phenolic recovery and antioxidant potential across all plants. Notably, exhibited the highest TPC (53.7 mg GAE/g) and strongest antioxidant activity (IC50 29.8 mg extract/g) under 200 W MW, 450 W US, and 12 min, yielding 14.5%. Rosemary achieved the highest yield (23.36%) with a TPC of 26.35 mg GAE/g and an IC50 of 40.75 mg extract/g at 200 W MW, 700 W US, and 8 min. Oregano's optimal conditions (500 W MW, 700 W US, 12 min) produced a TPC of 34.99 mg GAE/g and an IC50 of 50.31 mg extract/g. Chamomile extracts demonstrated lower phenolic content and antioxidant activity but achieved significant yields under 500 W MW, 700 W US, and 5 min. This study highlights UAE-MAE's superior efficiency, showcasing its potential to maximize phenolic recovery sustainably, making it a promising technique for industrial and therapeutic applications.

摘要

本研究提出了超声辅助和微波辅助萃取（UAE-MAE）的协同应用，作为一种从药用植物牛至、迷迭香和洋甘菊中提取生物活性化合物的新颖且高效的方法。以乙醇为溶剂，采用响应面法（RSM）对微波（MW）功率、超声（US）功率和萃取时间等萃取参数进行了优化。通过福林-西奥尔特法测定提取物的总酚含量（TPC），并使用DPPH法测定抗氧化活性（IC50）。采用二极管阵列检测的高效液相色谱法（HPLC-DAD）鉴定了对其抗氧化和治疗潜力有贡献的主要生物活性化合物。优化后的UAE-MAE条件提高了所有植物中酚类物质的回收率和抗氧化潜力。值得注意的是，在200 W MW、450 W US和12分钟的条件下，[此处原文缺失一种植物名称]表现出最高的TPC（53.7 mg GAE/g）和最强的抗氧化活性（IC50为29.8 mg提取物/g），产率为14.5%。迷迭香在200 W MW、700 W US和8分钟的条件下，产率最高（23.36%），TPC为26.35 mg GAE/g，IC50为40.75 mg提取物/g。牛至的最佳条件（500 W MW、700 W US、12分钟）产生的TPC为34.99 mg GAE/g，IC50为50.31 mg提取物/g。洋甘菊提取物的酚类含量和抗氧化活性较低，但在500 W MW、700 W US和5分钟的条件下获得了显著的产率。本研究突出了UAE-MAE的卓越效率，展示了其可持续最大化酚类回收率的潜力，使其成为工业和治疗应用中有前景的技术。