Kim Sun Young, Moon Hanbi, Kwon Yong Min, Kim Kyung Woo, Kim Jaoon Young Hwan
National Marine Biodiversity Institute of Korea, Jangsan-ro 101-75, Seocheon 33662, Republic of Korea.
Mar Drugs. 2024 Dec 18;22(12):567. doi: 10.3390/md22120567.
is well known for its potential for biofuel production due to its high lipid content. Numerous omics and biochemical studies have explored the overall molecular mechanisms underlying the responses of sp. to nutrient availability, primarily focusing on lipid metabolism. However, is able to synthesize other valuable products such as carotenoids, including violaxanthin, which has various biological functions and applications. In this study, we comparatively investigated the physiological, biochemical, and molecular responses of to nitrogen and phosphorus limitation, examining biomass production, photosynthetic activity, lipid, chlorophyll, and carotenoids content, and RNA-seq data. Nitrogen limitation decreased photosynthetic activity, chlorophyll content, and biomass production but increased lipid content. Phosphorus limitation substantially increased carotenoids content, with violaxanthin productivity of 10.24 mg/L, 3.38-fold greater than under the control condition, with little effect on biomass production or photosynthetic function. These results were generally consistent with the gene expression pattern observed in transcriptomic analysis. This integrated analysis shows that phosphorus limitation can be an economically competitive solution by enhancing valuable carotenoids while maintaining lipid and biomass production in .
因其高脂质含量在生物燃料生产方面具有潜力而闻名。众多组学和生化研究探索了 种对养分可利用性响应的整体分子机制，主要聚焦于脂质代谢。然而， 能够合成其他有价值的产物，如类胡萝卜素，包括具有多种生物学功能和应用的紫黄质。在本研究中，我们比较研究了 对氮和磷限制的生理、生化和分子响应，检测了生物量生产、光合活性、脂质、叶绿素和类胡萝卜素含量以及RNA测序数据。氮限制降低了光合活性、叶绿素含量和生物量生产，但增加了脂质含量。磷限制显著增加了类胡萝卜素含量，紫黄质产量为10.24 mg/L，比对照条件下高3.38倍，对生物量生产或光合功能影响较小。这些结果与转录组分析中观察到的基因表达模式总体一致。这种综合分析表明，磷限制通过在维持 中的脂质和生物量生产的同时提高有价值的类胡萝卜素含量，可能是一种具有经济竞争力的解决方案。
