Shlykov Mikhail A, Smirnova Polina V, Senotov Anatoliy S, Teterina Anastasia Yu, Minaychev Vladislav V, Smirnov Igor V, Novikov Roman A, Marchenko Ekaterina I, Salynkin Pavel S, Komlev Vladimir S, Fadeev Roman S, Fadeeva Irina S
Baikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences, Leninskiy Prospect 49, 119334 Moscow, Russia.
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, 142290 Moscow, Russia.
J Funct Biomater. 2024 Dec 6;15(12):368. doi: 10.3390/jfb15120368.
One of the key factors of the interaction 'osteoplastic material-organism' is the state of the implant surface. Taking into account the fact that the equilibrium in regeneration conditions is reached only after the reparative histogenesis process is completed, the implant surface is constantly modified. This work is devoted to the numerical description of the dynamic bilateral material-medium interaction under close to physiological conditions, as well as to the assessment of the comparability of the model with and experimental results. The semi-empirical model obtained on the basis of chemical kinetics allows us to describe numerically the processes occurring in the systems and extrapolates well to assess the behavior of dicalcium phosphate dihydrate (DCPD) material under conditions of ectopic (subcutaneous) implantation in Wistar rats. It is shown that an experiment conducted using a perfusion-diffusion bioreactor in a cell culture medium with the addition of fetal bovine serum (FBS) allows for achieving morphologically and chemically identical changes in the surface of the material in comparison with the real organism. This fact opens up wide possibilities for the creation of an analog of a 'laboratory-on-a-chip' and the transition from classical models to more controlled and mathematically based systems.
“骨塑性材料 - 生物体”相互作用的关键因素之一是植入物表面的状态。考虑到只有在修复性组织发生过程完成后才会在再生条件下达到平衡这一事实，植入物表面会不断发生变化。这项工作致力于在接近生理条件下对动态双边材料 - 介质相互作用进行数值描述，以及评估该模型与实验结果的可比性。基于化学动力学获得的半经验模型使我们能够对系统中发生的过程进行数值描述，并能很好地外推以评估二水磷酸二钙（DCPD）材料在Wistar大鼠异位（皮下）植入条件下的行为。结果表明，在添加胎牛血清（FBS）的细胞培养基中使用灌注 - 扩散生物反应器进行的实验，与真实生物体相比，能够使材料表面在形态和化学上发生相同的变化。这一事实为创建“芯片实验室”类似物以及从经典模型向更可控且基于数学的系统转变开辟了广阔的可能性。
