Ismaiel Adnan, Jambhulkar Prashant P, Sinha Parimal, Lakshman Dilip K
Environmental Microbial & Food Safety Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA.
Department of Plant Pathology, Rani Lakshmi Bai Central Agricultural University, Jhansi 284003, India.
J Fungi (Basel). 2024 Nov 23;10(12):813. doi: 10.3390/jof10120813.
As environmental and health concerns increase, the trend toward sustainable agriculture is moving toward using biological agents. About 60% of all biological fungicides have species as the active ingredient, with as the most common species in these products. However, the name has often been used incorrectly in culture collections, databases, and scientific literature due to the division of the Harzianum clade (HC) into more than 95 cryptic species, with only one being named . In this study, 49 strains previously identified as in three surveys of species from soils in South and Central America were re-identified using phylogenetic analyses based on , , and ITS sequences obtained from GenBank. These were combined with the HC species from two other studies, which were identified based on the current taxonomy. Based on the results of the five surveys of the total 148 strains in HC, 11 species were identified. , and , followed by and , were the dominant species of the HC in South and Central America. This is the first report to identify dominant species within the HC in South and Central American soil based on multiple studies. These results will be useful in selecting strains within the clade for the formulation of biocontrol and biofertilizer products on the continent.
随着环境和健康问题日益受到关注，可持续农业的发展趋势正朝着使用生物制剂的方向发展。在所有生物杀菌剂中，约60%以菌种作为活性成分，其中 是这些产品中最常见的菌种。然而，由于哈茨木霉进化枝（HC）被划分为95个以上的隐种，其中只有一个被命名为 ，因此该名称在菌种保藏中心、数据库和科学文献中经常被错误使用。在本研究中，利用从GenBank获得的 、 和ITS序列进行系统发育分析，对之前在南美洲和中美洲土壤中进行的三次 菌种调查中鉴定为 的49个菌株进行了重新鉴定。这些菌株与另外两项研究中的HC菌种相结合，后者是根据当前的分类法鉴定的。基于对HC中总共148个菌株的五次调查结果，鉴定出了11个菌种。在南美洲和中美洲， 、 和 是HC中的优势菌种，其次是 和 。这是第一份基于多项研究确定南美洲和中美洲土壤中HC内优势 菌种的报告。这些结果将有助于在该进化枝内选择菌株，用于在该大陆配制生物防治和生物肥料产品。
