Embrapa Recursos Genéticos e Biotecnologia, Brasília, Brazil.
PLoS One. 2020 Mar 4;15(3):e0228485. doi: 10.1371/journal.pone.0228485. eCollection 2020.
Fifty four Trichoderma strains were isolated from soil samples collected from garlic and onion crops in eight different sites in Brazil and were identified using phylogenetic analysis based on combined ITS region, tef1-α, cal, act and rpb2 sequences. The genetic variability of the recovered Trichoderma species was analysed by AFLP and their phenotypic variability determined using MALDI-TOF. The strain clusters from both typing techniques coincided with the taxonomic determinations made from phylogenetic analysis. The phylogenetic analysis showed the occurrence of Trichoderma asperellum, Trichoderma asperelloides, Trichoderma afroharzianum, Trichoderma hamatum, Trichoderma lentiforme, Trichoderma koningiopsis, Trichoderma longibrachiatum and Trichoderma erinaceum, in the soil samples. We also identified and describe two new Trichoderma species, both in the harzianum clade of section Pachybasium, which we have named Trichoderma azevedoi sp. nov. and Trichoderma peberdyi sp. nov. The examined strains of both T. azevedoi (three strains) and T. peberdyi (12 strains) display significant genotypic and phenotypic variability, but form monophyletic clades with strong bootstrap and posterior probability support and are morphologically distinct from their respective most closely related species.
从巴西八个不同地点的大蒜和洋葱作物土壤样本中分离出 54 株木霉菌株，通过基于 ITS 区、tef1-α、cal、act 和 rpb2 序列的系统发育分析进行鉴定。利用 AFLP 分析了回收的木霉物种的遗传变异性，并利用 MALDI-TOF 测定其表型变异性。两种分型技术的菌株聚类与系统发育分析的分类学测定结果一致。系统发育分析显示，土壤样本中存在asperellum、asperelloides、afroharzianum、hamatum、lentiforme、koningiopsis、longibrachiatum 和 erinaceum 等木霉属。我们还鉴定并描述了两种新的木霉属物种，均属于 Pachybasium 节的 harzianum 分支，分别命名为 Trichoderma azevedoi sp. nov. 和 Trichoderma peberdyi sp. nov. 研究的 T. azevedoi（三株）和 T. peberdyi（12 株）菌株表现出显著的遗传和表型变异性，但形成单系聚类，具有较强的自举和后验概率支持，并且在形态上与各自最密切相关的物种明显不同。
