Wu Na, Li Zhen, Wu Fei, Tan Jing
Institute of Applied Biotechnology, College of Agronomy and Life Sciences, Shanxi Datong University, Datong 037009, China.
College of Life Sciences, Northwest Normal University, Lanzhou 730070, China.
J Fungi (Basel). 2024 Nov 26;10(12):822. doi: 10.3390/jof10120822.
Native ectomycorrhizal fungi (ECMF) are generally more effective than non-native ECMF in facilitating the phytoremediation of heavy metal (HM) ions from contaminated soils. This study aimed to investigate the contributions of four ECMF species-, , , and -that were isolated from mining areas to the growth, water status, photosynthesis, and metallothionein gene expression of exposed to varying concentrations of lead (Pb). The experiment lasted two months and involved . cuttings subjected to Pb concentrations of 0, 200, and 400 mg kg, representing no Pb stress, moderate Pb stress, and severe Pb stress, respectively. Results indicated that . significantly enhanced the growth, water status, photosynthesis parameters, and metallothionein gene expression of . under Pb stress, whereas . only exhibited positive effects under severe Pb stress. . negatively affected the growth, water status, photosynthesis, and metallothionein gene expression of . under Pb stress, while no significant difference was observed between the control treatment and . symbiosis. Therefore, . and . are promising ECMF species for ecological restoration in mining areas, especially in . woodlands.
本地外生菌根真菌（ECMF）在促进从污染土壤中植物修复重金属（HM）离子方面通常比非本地ECMF更有效。本研究旨在调查从矿区分离出的四种ECMF物种——、、和——对暴露于不同浓度铅（Pb）下的的生长、水分状况、光合作用和金属硫蛋白基因表达的贡献。实验持续了两个月，涉及。插条分别经受0、200和400 mg kg的Pb浓度，分别代表无Pb胁迫、中度Pb胁迫和重度Pb胁迫。结果表明，。显著增强了Pb胁迫下的生长、水分状况、光合作用参数和金属硫蛋白基因表达，而。仅在重度Pb胁迫下表现出积极影响。。在Pb胁迫下对的生长、水分状况、光合作用和金属硫蛋白基因表达产生负面影响，而在对照处理和。共生之间未观察到显著差异。因此，。和。是矿区生态恢复的有前景的ECMF物种，特别是在。林地。
