Xiao Xiong, Liu Quan, Zhang Qian, Yan Zhenzhen, Cai Dongbo, Li Xin
Cooperative Innovation Center of Industrial Fermentation (Ministry of Education and Hubei Province), Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Key Laboratory of Industrial Microbiology, School of Life and Health Sciences, Hubei University of Technology, Wuhan 430068, China.
State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430068, China.
J Fungi (Basel). 2024 Dec 5;10(12):842. doi: 10.3390/jof10120842.
is a typical aroma-producing yeast in food brewing, but it has low heat resistance and poor proliferation ability at high temperature. Trehalose is generally considered to be a protective agent that helps stable yeast cells resist heat shock stress, but its functional mechanism for yeast cells in the adaptation period under heat stress is unclear. In this study, the physiological metabolism changes, specific gene transcription expression characteristics, and transcriptome differences of under different carbon sources under high-temperature stress (40 °C) were compared to explore the mechanism of trehalose inducing to recover and proliferate under high-temperature stress during the adaptation period. The results showed that high concentration of trehalose (20% Tre) could not be used as the main carbon source for the proliferation of under long-term high-temperature stress, but it helped to maintain the stability of the cell population. The intracellular trehalose of was mainly derived from the synthesis and metabolism of intracellular glucose, and the extracellular acetic acid concentration showed an upward trend with the improvement of yeast growth. A high concentration of trehalose (20% Tre) can promote the expression of high glucose receptor gene (12.0-fold) and induce the up-regulation of (27.1-fold), (58.9-fold), (8.3-fold), and other signal transduction protein genes, and the increase of trehalose concentration will maintain the temporal up-regulation of these genes. Transcriptome analysis showed that trehalose concentration and the presence of glucose had a significant effect on the gene expression of under high-temperature stress. In summary, trehalose assists in adapting to high temperature by changing gene expression levels, and assists in absorbing glucose to achieve cell proliferation.
是食品酿造中一种典型的产香酵母，但它耐热性低，在高温下增殖能力差。海藻糖通常被认为是一种保护剂，有助于稳定酵母细胞抵抗热激胁迫，但其在热胁迫适应期对酵母细胞的功能机制尚不清楚。本研究比较了高温胁迫（40℃）下不同碳源条件下的生理代谢变化、特定基因转录表达特征及转录组差异，以探讨海藻糖诱导在适应期高温胁迫下恢复和增殖的机制。结果表明，高浓度海藻糖（20% Tre）在长期高温胁迫下不能作为增殖的主要碳源，但有助于维持细胞群体的稳定性。的细胞内海藻糖主要来源于细胞内葡萄糖的合成与代谢，细胞外乙酸浓度随酵母生长的改善呈上升趋势。高浓度海藻糖（20% Tre）可促进高葡萄糖受体基因（12.0倍）的表达，并诱导（27.1倍）、（58.9倍）、（8.3倍）等信号转导蛋白基因上调，海藻糖浓度的增加将维持这些基因的时间上调。转录组分析表明，海藻糖浓度和葡萄糖的存在对高温胁迫下的基因表达有显著影响。综上所述，海藻糖通过改变基因表达水平协助适应高温，并协助吸收葡萄糖以实现细胞增殖。
