Rinaldi Valentina, Bongiovanni Laura, Crisi Paolo Emidio, Vignoli Massimo, Peli Renato Ennio, Masci Stefano, Boari Andrea, Finotello Riccardo
Department of Veterinary Medicine, University of Teramo, 64100 Teramo, Italy.
Department of Biomolecular Sciences, Faculty of Veterinary Medicine, Utrecth University, 3584 CS Utrecht, The Netherlands.
Vet Sci. 2024 Dec 6;11(12):628. doi: 10.3390/vetsci11120628.
An 11-year-old spayed female Beagle presented with tenesmus and was identified with a rectal wall mass. Diagnostic imaging (abdominal ultrasound and computed tomography) localised the mass in the right rectal wall and documented no evidence of metastatic disease. Subsequently, the dog underwent surgery for tumour excision. A histopathological diagnosis of melanoma was performed. To confirm the tumour histotype, immunohistochemistry was performed using anti-Melan A and anti-Ki67. Neoplastic cells exhibited focal Melan A immunoreactivity and widespread nuclear immunoreactivity for Ki67 with a Ki67 index of 27%. Adjuvant immunotherapy with APAVAC was initiated. After APAVAC administration, no local or systemic acute adverse events were observed. Four pre- and post-contrast computed tomography (CT) studies were performed in an 18-month follow-up period every 4-5 months. Follow-up rectal palpation and conscious visualisation of the surgical site have also resulted in no macroscopic signs of tumour recurrence. The dog remains alive and with no clinical evidence of tumour recurrence and/or distant progression at the time of writing, therefore, surviving over 540 days from the diagnosis.
一只11岁已绝育的雌性比格犬出现里急后重症状，经检查发现直肠壁有肿物。诊断性影像学检查（腹部超声和计算机断层扫描）确定肿物位于直肠右壁，且未发现转移病灶的证据。随后，该犬接受了肿瘤切除手术。进行了黑色素瘤的组织病理学诊断。为确认肿瘤组织类型，使用抗Melan A和抗Ki67进行了免疫组织化学检测。肿瘤细胞显示出局灶性Melan A免疫反应性，Ki67呈广泛的核免疫反应性，Ki67指数为27%。开始使用APAVAC进行辅助免疫治疗。给予APAVAC后，未观察到局部或全身急性不良事件。在18个月的随访期内，每4至5个月进行4次增强前后计算机断层扫描（CT）检查。随访时直肠触诊及对手术部位的直观观察均未发现肿瘤复发的宏观迹象。在撰写本文时，该犬仍然存活，且无肿瘤复发和/或远处进展的临床证据，因此，自诊断以来已存活超过540天。
