He Mengqian, Xu Jiehuan, Sun Lingwei, Wu Caifeng, Zhang Shushan, Gao Jun, Zhang Defu, Dai Jianjun
Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
Key Laboratory of Livestock and Poultry Resources (Pig) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Shanghai 201106, China.
Vet Sci. 2024 Dec 13;11(12):647. doi: 10.3390/vetsci11120647.
There are many applications of soybean lecithin (SL) and cholesterol-loaded cyclodextrin (CLC) in sperm freezing processes. To the best of our knowledge, there have been few cases of the combined use of SL and CLC in freezing rooster semen. We investigated the effects of CLC, SL, and their combination on rooster sperm cryodamage. Three experiments were conducted: experiment 1, SL (0.5%, 1%, 1.5%, 2.0%); experiment 2, CLC (1.25 mg, 2.5 mg, 5 mg, 10 mg, 15 mg); and experiment 3, CLC + SL (2.5 mg + 0.25%, 2.5 mg + 0.5%, 2.5 mg + 1%, 2.5 mg + 1.5%). Semen samples were cryopreserved using a programmed cryostat, followed by the determination of post-thaw sperm quality, antioxidant indices, and hatching. The results showed that the combination of 2.5 mg CLC + 0.5% SL had the most significant synergistic effect on cryodamage, and the viability (56.69%), motility (54.35%), mitochondrial activity (54.23%), plasma membrane integrity (53.52%), acrosome integrity (54.71%), and antioxidant activity (MDA concentration: 5.65 nmol/mL; SOD activity: 152.73 U/mL) were significantly greater than those of the other combinations ( < 0.05). Nevertheless, the combined CLC and SL addition group did not substantially increase the fertilization and hatching rates of frozen semen compared with the addition of 2.5 mg CLC. In conclusion, the addition of 2.5 mg CLC and 2.5 mg CLC + 0.5% SL enhanced the quality and fertility of frozen rooster sperm.
大豆卵磷脂（SL）和胆固醇负载环糊精（CLC）在精子冷冻过程中有许多应用。据我们所知，在冷冻公鸡精液中联合使用SL和CLC的情况很少。我们研究了CLC、SL及其组合对公鸡精子冷冻损伤的影响。进行了三个实验：实验1，SL（0.5%、1%、1.5%、2.0%）；实验2，CLC（1.25毫克、2.5毫克、5毫克、10毫克、15毫克）；实验3，CLC + SL（2.5毫克 + 0.25%、2.5毫克 + 0.5%、2.5毫克 + 1%、2.5毫克 + 1.5%）。使用程序降温仪对精液样本进行冷冻保存，随后测定解冻后精子质量、抗氧化指标和孵化率。结果表明，2.5毫克CLC + 0.5% SL组合对冷冻损伤具有最显著的协同作用，其活力（56.69%）、运动能力（54.35%）、线粒体活性（54.23%）、质膜完整性（53.52%）、顶体完整性（54.71%）和抗氧化活性（丙二醛浓度：5.65纳摩尔/毫升；超氧化物歧化酶活性：152.73单位/毫升）显著高于其他组合（<0.05）。然而，与添加2.5毫克CLC相比，联合添加CLC和SL组并未显著提高冷冻精液的受精率和孵化率。总之，添加2.5毫克CLC和2.5毫克CLC + 0.5% SL可提高冷冻公鸡精子的质量和受精能力。
