College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
Cryobiology. 2019 Aug;89:90-95. doi: 10.1016/j.cryobiol.2019.05.001. Epub 2019 May 2.
This is a unique study because is the first time we are adding melatonin into an extender in order to determine its influence on cryopreserved chicken semen. The primary focus of our present study was to evaluate the influence of different concentrations of Melatonin on cryopreserved chicken semen. Semen samples were allocated into four treatments, being one control and three different combinations of antioxidants and after the freeze-thaw operation, the sperm motility, plasma membrane integrity, acrosome integrity, endogenous enzymes (GSH-Px, CAT, SOD), MDA and ROS of chicken spermatozoa were all evaluated. The collection of the semen samples was from 40 Arbor Acre roosters and this procedure was repeated twice a week and then mixed in an extender that contained different MEL treatments as follows: a diluent without MEL (control, M 0), a diluent comprising 0.125 mg/mL (M 0.125) 0.25 mg/mL, (M 0.25) and 0.5 mg/mL (M 0.5). It was revealed that the supplementation of the base extender with an optimal 0.25 mg/mL MEL led to a higher significant difference in the motility of chicken sperm (P < 0.01), higher acrosome integrity (P < 0.05) and a higher plasma membrane integrity (P < 0.01) when compared to the control group at post-thaw. Furthermore, when compared to the control group, 0.25 mg/mL MEL addition into the extender significantly enhanced the activity of endogenous enzymes (GSH-Px, CAT, and SOD) in the chicken spermatozoa at post-thaw (P < 0.05). Moreover, 0.5 mg/mL MEL supplementation into the extender enhanced the GSH-Px activity in the chicken spermatozoa when compared with the control group (P < 0.05) at post-thaw. In contrast, the addition of 0.25 mg/mL MEL into the extender resulted in a significantly lower MDA in comparison to the 0.125 mg/mL, 0.5 mg/mL MEL treatment group and the control group (P < 0.05). Also, compared to the control group, MEL concentration of 0.125 mg/mL and 0.5 mg/mL MEL into the extender resulted in a significantly low ROS concentration (P < 0.05) but the addition of 0.25 mg/mL MEL concentration resulted in a significantly lower ROS level when compared to the control group (P < 0.01). In summary, MEL improved the quality of cryopreserved chicken sperm quality by decreasing oxidative stress level and the most optimal concentration was 0.25 mg/mL.
这是一项独特的研究，因为这是我们第一次在延长器中添加褪黑素，以确定其对冷冻鸡精液的影响。我们目前研究的主要重点是评估不同浓度的褪黑素对冷冻鸡精液的影响。将精液样本分配到四个处理组中，其中一组为对照组，另外三组为不同浓度的抗氧化剂组合，然后进行冻融操作，评估鸡精子的运动能力、质膜完整性、顶体完整性、内源性酶（GSH-Px、CAT、SOD）、MDA 和 ROS。精液样本来自 40 只 Arbor Acre 公鸡，每周重复两次采集样本，然后将其混合到含有不同 MEL 处理的延长剂中，具体如下：不含 MEL 的稀释剂（对照组，M0）、0.125mg/ml（M0.125）、0.25mg/ml（M0.25）和 0.5mg/ml（M0.5）。结果表明，与对照组相比，基础延长剂中添加最佳浓度 0.25mg/ml 的 MEL 可显著提高鸡精子的运动能力（P<0.01），显著提高顶体完整性（P<0.05）和质膜完整性（P<0.01）。此外，与对照组相比，在冻融后，在延长剂中添加 0.25mg/ml 的 MEL 可显著提高鸡精子中的内源性酶（GSH-Px、CAT 和 SOD）的活性（P<0.05）。此外，与对照组相比，在冻融后，在延长剂中添加 0.5mg/ml 的 MEL 可显著提高鸡精子中的 GSH-Px 活性（P<0.05）。相比之下，与对照组相比，在延长剂中添加 0.25mg/ml 的 MEL 可显著降低 MDA 水平，与 0.125mg/ml、0.5mg/ml MEL 处理组和对照组相比（P<0.05）。此外，与对照组相比，在延长剂中添加 0.125mg/ml 和 0.5mg/ml MEL 浓度可显著降低 ROS 浓度（P<0.05），但与对照组相比，在延长剂中添加 0.25mg/ml MEL 浓度可显著降低 ROS 水平（P<0.01）。总之，褪黑素通过降低氧化应激水平来提高冷冻鸡精子的质量，最佳浓度为 0.25mg/ml。
