Ferreira Henrique Rafael Pontes, de Moura Barbosa Taciano, Vasconcelos Simão Dias
Laboratory of Insects of Forensic Importance, Department of Zoology, Universidade Federal de Pernambuco, Recife, Brazil.
Med Vet Entomol. 2025 Mar;39(1):78-88. doi: 10.1111/mve.12784. Epub 2024 Dec 27.
The nocturnal behaviour and reproduction patterns of Sarcophagidae species during the scotophase are largely unexplored for species in the Neotropical region. The aim of this study was to assess the light regimes under which females of Peckia (Peckia) chrysostoma (Wiedemann, 1830) and Peckia (Sarcodexia) lambens (Wiedemann, 1830) would larviposit and understand how these variables influence intrauterine development in these flies. The experiments were conducted in an experimental room (lux = 100) in two independent experiments: I. Larviposition test: Females mated with males of over 8 days old. II. Intrauterine development test: females over 8 days old and mated were individually isolated. Both experiments were performed under photophases (L:D) of 0:24, 6:18; 12:12; 18:6 and 24:0 for a period of 96 h. Larviposition occurred in total darkness for P. (P.) chrysostoma and P. (S.) lambens in all treatments, without significant differences among photophase treatments (p > 0.05). Photophase influenced the timing of larviposition, occurring as early as 24 h in 12:12 L:D conditions, while no larviposition was observed in the first 24 h in the 0:24 treatment for either species. The light duration significantly affected the percentage of gravid females of both species (p < 0.05), with higher percentages of gravid females in treatments of longer light duration. Females at the initial and intermediate stages of egg development were more strongly associated with treatments of continuous darkness (0:24) or short photophase (6:18), whereas females with an advanced stage of egg development were more prevalent in the 12:12 treatment. The implications of these findings for forensic entomology are profound, challenging conventional knowledge by revealing that necrophagous insects are not limited to diurnal activity patterns.
对于新热带地区的麻蝇科物种而言，其在暗期的夜间行为和繁殖模式在很大程度上尚未得到探索。本研究的目的是评估金黄皮蠹（Peckia (Peckia) chrysostoma (Wiedemann, 1830)）和兰氏皮蠹（Peckia (Sarcodexia) lambens (Wiedemann, 1830)）雌蝇进行幼虫产出的光照条件，并了解这些变量如何影响这些苍蝇的子宫内发育。实验在一个实验室内（照度 = 100）进行了两个独立实验：I. 幼虫产出试验：雌蝇与8日龄以上的雄蝇交配。II. 子宫内发育试验：8日龄以上且已交配的雌蝇单独隔离。两个实验均在光周期（L:D）为0:24、6:18、12:12、18:6和24:0的条件下进行96小时。在所有处理中，金黄皮蠹和兰氏皮蠹的幼虫产出均发生在完全黑暗中，光周期处理之间无显著差异（p > 0.05）。光周期影响幼虫产出的时间，在12:12 L:D条件下最早在24小时出现幼虫产出，而在0:24处理中，两种物种在最初24小时内均未观察到幼虫产出。光照时长显著影响两种物种的怀孕雌蝇百分比（p < 0.05），光照时长较长的处理中怀孕雌蝇百分比更高。处于卵发育初始和中间阶段的雌蝇与持续黑暗（0:24）或短光周期（6:18）处理的关联更强，而处于卵发育后期的雌蝇在12:12处理中更为普遍。这些发现对法医昆虫学具有深远影响 通过揭示食尸性昆虫不限于日间活动模式，挑战了传统认知。
