Whisson Stephen C, Welsh Lydia R J, Vetukuri Ramesh R
Cell and Molecular Sciences Department, The James Hutton Institute, Invergowrie, Dundee, UK.
Department of Plant Breeding, Swedish University of Agricultural Sciences (SLU), Lomma, Sweden.
Methods Mol Biol. 2025;2892:23-34. doi: 10.1007/978-1-0716-4330-3_2.
RNA silencing is a core cellular process that acts to defend the genome against potentially damaging genetic elements such as viruses and transposons. It has been extensively characterized in many eukaryotes and exploited as a tool for determining gene function through removing the activity of specific genes. It has also been used in Phytophthora species to reveal genes involved in different lifecycle stages. In this chapter, we provide guidelines and outline considerations for carrying out RNA silencing experiments in Phytophthora.
RNA沉默是一种核心的细胞过程，其作用是保护基因组免受病毒和转座子等潜在有害遗传元件的侵害。它已在许多真核生物中得到广泛表征，并被用作通过去除特定基因的活性来确定基因功能的工具。它也已被用于疫霉属物种中，以揭示参与不同生命周期阶段的基因。在本章中，我们提供了在疫霉中进行RNA沉默实验的指导方针并概述了注意事项。
