College of Plant Protection, China Agricultural University, Beijing, 100193, China.
College of Plant Protection, Academy for Advanced Interdisciplinary Studies, Nanjing Agricultural University, Nanjing, 210095, China.
Nat Commun. 2023 Sep 12;14(1):5622. doi: 10.1038/s41467-023-41447-8.
Potato late blight caused by Phytophthora infestans is a devastating disease worldwide. Unlike other plant pathogens, double-stranded RNA (dsRNA) is poorly taken up by P. infestans, which is a key obstacle in using dsRNA for disease control. Here, a self-assembled multicomponent nano-bioprotectant for potato late blight management is designed based on dsRNA and a plant elicitor. Nanotechnology overcomes the dsRNA delivery bottleneck for P. infestans and extends the RNAi protective window. The protective effect of nano-enabled dsRNA against infection arises from a synergistic mechanism that bolsters the stability of dsRNA and optimizes its effective intracellular delivery. Additionally, the nano-enabled elicitor enhances endocytosis and amplifies the systemic defense response of the plants. Co-delivery of dsRNA and an elicitor provides a protective effect via the two aspects of pathogen inhibition and elevated plant defense mechanisms. The multicomponent nano-bioprotectant exhibits superior control efficacy compared to a commercial synthetic pesticide in field conditions. This work proposes an eco-friendly strategy to manage devastating plant diseases and pests.
由致病疫霉引起的马铃薯晚疫病是一种全球性的破坏性疾病。与其他植物病原体不同，双链 RNA（dsRNA）在致病疫霉中摄取不良，这是利用 dsRNA 进行疾病控制的一个关键障碍。在这里，基于 dsRNA 和植物激发子设计了一种用于马铃薯晚疫病管理的自组装多组分纳米生物保护剂。纳米技术克服了 dsRNA 对 P. infestans 的输送瓶颈，并延长了 RNAi 的保护窗口。纳米增强 dsRNA 对感染的保护作用源于一种协同机制，该机制增强了 dsRNA 的稳定性并优化了其有效的细胞内传递。此外，纳米增强型激发子增强了内吞作用，并放大了植物的系统防御反应。dsRNA 和激发子的共递送通过抑制病原体和提高植物防御机制的两个方面提供了保护作用。多组分纳米生物保护剂在田间条件下表现出比商业合成农药更高的控制效果。这项工作提出了一种管理破坏性植物病虫害的环保策略。
