Palmer Jade T T, Gerth Monica L
School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand.
Methods Mol Biol. 2025;2892:139-149. doi: 10.1007/978-1-0716-4330-3_10.
Here, we present a protocol for the isolation and detection of Phytophthora oospores directly from soil samples. Our method incorporates a novel technique for isolating Phytophthora oospores using filter pouches and an improved DNA extraction procedure specifically designed for oospores. While we have primarily developed this protocol for detecting P. agathidicida oospores using end-point PCR, we believe these methods can be readily adapted for other Phytophthora species. Furthermore, the DNA extracted using this protocol is suitable as input for other DNA-based detection methods.
在此，我们展示了一种直接从土壤样本中分离和检测疫霉卵孢子的方案。我们的方法纳入了一项使用滤袋分离疫霉卵孢子的新技术，以及一种专门为卵孢子设计的改进型DNA提取程序。虽然我们主要是为了通过终点PCR检测阿加西疫霉卵孢子而开发此方案，但我们相信这些方法可轻松适用于其他疫霉物种。此外，使用此方案提取的DNA适合作为其他基于DNA的检测方法的输入。
