Andronis Christina E, Bringans Scott, Tan Kar-Chun
Proteomics International, Nedlands, WA, Australia.
The Centre for Crop and Disease Management, Curtin University, Bentley, WA, Australia.
Methods Mol Biol. 2025;2892:211-231. doi: 10.1007/978-1-0716-4330-3_15.
The biochemical makeup of any organism provides insight into key factors regarding its biological functions. These factors can be explored using proteomics, which allows us to obtain a snapshot of the protein content and abundance in an organism, cell type or sub-cellular compartment. Here, we describe proteomic methodologies that can be used to dissect the biochemical mechanism of phytopathogenicity in oomycetes. These methodologies include protein extraction, purification, subsequent processing, mass spectrometry analysis, and qualitative and quantitative data processing of oomycete proteomes for comparative studies. Additionally, the use of mass spectra to assist in gene validation and modelling in unfinished oomycete genomes is also described.
任何生物体的生化组成都能为了解其生物学功能的关键因素提供线索。这些因素可以通过蛋白质组学来探究，蛋白质组学使我们能够获取生物体、细胞类型或亚细胞区室中蛋白质的含量和丰度的快照。在此，我们描述了可用于剖析卵菌中植物致病性生化机制的蛋白质组学方法。这些方法包括蛋白质提取、纯化、后续处理、质谱分析，以及对卵菌蛋白质组进行定性和定量数据处理以进行比较研究。此外，还介绍了利用质谱来辅助未完成的卵菌基因组中的基因验证和建模。
