Elekhtiar Sally A, Abo Gazia Maha M, Osman Amira, Abd-Elsalam Marwa M, El-Kemary Nesma M, Elksass Samar, Alkabes Hend A, El-Kemary Maged
Department of Histology, Faculty of Medicine, Kafrelsheikh University, Kafr ElSheikh, 33516, Egypt.
Department of Histology, Faculty of Medicine, Kafrelsheikh University, Kafr ElSheikh, 33516, Egypt; Department of Basic Medical and Dental Sciences, Faculty of Dentistry, Zarqa University, Zarqa, 13132, Jordan.
J Tissue Viability. 2025 Feb;34(1):100850. doi: 10.1016/j.jtv.2024.12.014. Epub 2024 Dec 24.
Despite the advances in the development of therapeutic wearable wound-healing patches, lack self-healing properties and strong adhesion to diabetic skin, hindering their effectiveness. We propose a unique, wearable patch made from a 3D organo-hydrogel nanocomposite containing polydopamine, titanium dioxide nanoparticles, and silver quantum dots (PDA-TiO@Ag). The designed patch exhibits ultra-stretchable, exceptional-self-healing, self-adhesive, ensuring conformal contact with the skin even during movement. Our patch demonstrated potent antibacterial activity and significantly accelerated wound healing with a high wound closure rate of 99.2 % after 7 days. Remarkably, it enhanced diabetic skin wound healing compared to that achieved by adipose-derived stem cell (ADSC) therapy in a study involving 30 adult male albino rats. Microscopic analysis highlights the promising hierarchical architecture structure of the patch for wound healing applications, suggesting its potential to create a favorable environment for healing and provide long-lasting benefits. Histopathological analysis and immunohistochemical staining revealed faster healing and enhanced cellular response in the patch-treated group compared to both stem cell and control groups. Notably, the patch promoted complete re-epithelization and a significant increase in vascular endothelial growth factor (VEGF) expression on day 7, indicating improved angiogenesis. This self-healing, multifunctional patch offers a promising alternative to stem cell therapy for accelerating diabetic wound healing, showcasing its potential for clinical translation. The combination of durability, biocompatibility, and antibacterial properties makes the patch a promising candidate for advanced wound management and offering faster, more complete restoration than other approaches.
尽管治疗性可穿戴伤口愈合贴片在开发方面取得了进展，但缺乏自我愈合特性以及对糖尿病皮肤的强粘附性，这阻碍了它们的有效性。我们提出了一种独特的可穿戴贴片，它由包含聚多巴胺、二氧化钛纳米颗粒和银量子点（PDA-TiO@Ag）的3D有机水凝胶纳米复合材料制成。所设计的贴片具有超拉伸性、卓越的自我愈合性和自粘性，即使在运动过程中也能确保与皮肤紧密贴合。我们的贴片展现出强大的抗菌活性，并显著加速了伤口愈合，7天后伤口闭合率高达99.2%。值得注意的是，在一项涉及30只成年雄性白化大鼠的研究中，与脂肪来源干细胞（ADSC）疗法相比，它增强了糖尿病皮肤伤口的愈合。微观分析突出了该贴片在伤口愈合应用中具有前景的分级结构，表明其有潜力创造有利于愈合的环境并提供持久益处。组织病理学分析和免疫组织化学染色显示，与干细胞组和对照组相比，贴片治疗组的愈合更快且细胞反应增强。值得注意的是，贴片在第7天促进了完全重新上皮化，并显著增加了血管内皮生长因子（VEGF）的表达，表明血管生成得到改善。这种自我愈合的多功能贴片为加速糖尿病伤口愈合提供了一种有前景的替代干细胞疗法的方法，展示了其临床转化的潜力。耐用性、生物相容性和抗菌特性的结合使该贴片成为先进伤口管理的有前景候选者，并且比其他方法能提供更快、更完全的恢复。
