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RFC3基因敲低可降低宫颈癌细胞的增殖、迁移和侵袭能力。

RFC3 Knockdown Decreases Cervical Cancer Cell Proliferation, Migration and Invasion.

作者信息

Koh Jae Woong, Park Seon-Joo

机构信息

Ophthalmic and Optic Medical Device Globalization Team (Ministry of Trade, Industry, and Energy), Chosun University, Gwangju, Republic of Korea.

Department of Ophthalmology, College of Medicine, Chosun University, Gwangju, Republic of Korea.

出版信息

Cancer Genomics Proteomics. 2025 Jan-Feb;22(1):127-135. doi: 10.21873/cgp.20493.

DOI:10.21873/cgp.20493
PMID:39730174
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11696326/
Abstract

BACKGROUND/AIM: Replication factor C subunit 3 (RFC3) is a critical component of the replication factor C complex, which is essential for DNA replication and repair. Recent studies have highlighted the RFC3's significance in various cancer types. Herein, we aimed to elucidate its biological role in cervical cancer.

MATERIALS AND METHODS

Cervical cancer cells were transfected with RFC3 or control siRNA. Cell viability was assessed using the MTT assay over a 4-day period and its clonogenic potential was determined using colony formation assays. Flow cytometry analysis was performed to evaluate cell cycle distribution. Transwell migration and invasion assays were performed to assess the migration and invasion abilities of cervical cancer cells.

RESULTS

RFC3 knockdown significantly inhibited cell proliferation, induced cell-cycle arrest, and decreased migration and invasion in HeLa and ME-180 cells compared to control siRNA-transfected cells.

CONCLUSION

The crucial role of RFC3 in cervical cancer progression is highlighted. RFC3 knockdown resulted in decreased cervical cancer cell proliferation, migration and invasion, suggesting its potential as a therapeutic target in cervical cancer.

摘要

背景/目的：复制因子C亚基3（RFC3）是复制因子C复合物的关键组成部分，对DNA复制和修复至关重要。最近的研究突出了RFC3在多种癌症类型中的重要性。在此，我们旨在阐明其在宫颈癌中的生物学作用。

材料与方法

用RFC3或对照小干扰RNA转染宫颈癌细胞。在4天的时间内使用MTT法评估细胞活力，并使用集落形成试验确定其克隆形成潜力。进行流式细胞术分析以评估细胞周期分布。进行Transwell迁移和侵袭试验以评估宫颈癌细胞的迁移和侵袭能力。

结果

与对照小干扰RNA转染的细胞相比，RFC3基因敲低显著抑制HeLa和ME-180细胞的增殖，诱导细胞周期停滞，并降低迁移和侵袭能力。

结论

突出了RFC3在宫颈癌进展中的关键作用。RFC3基因敲低导致宫颈癌细胞增殖、迁移和侵袭减少，表明其作为宫颈癌治疗靶点的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebc7/11696326/1654f4a39090/cgp-22-133-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebc7/11696326/ac9239770418/cgp-22-128-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebc7/11696326/715778886264/cgp-22-129-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebc7/11696326/7b7931b905cb/cgp-22-130-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebc7/11696326/e8a812ef1408/cgp-22-131-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebc7/11696326/5c34d4a3aeb7/cgp-22-132-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebc7/11696326/1654f4a39090/cgp-22-133-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebc7/11696326/ac9239770418/cgp-22-128-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebc7/11696326/715778886264/cgp-22-129-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebc7/11696326/7b7931b905cb/cgp-22-130-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebc7/11696326/e8a812ef1408/cgp-22-131-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebc7/11696326/5c34d4a3aeb7/cgp-22-132-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebc7/11696326/1654f4a39090/cgp-22-133-g0001.jpg

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The pathophysiology of the cell cycle in cancer and treatment strategies using various cell cycle checkpoint inhibitors.
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Metastasis.转移。
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Cell cycle control in cancer.癌症中的细胞周期调控。
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Estimates of the global burden of cervical cancer associated with HIV.与 HIV 相关的宫颈癌全球负担估计。
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RFC3 induces epithelial‑mesenchymal transition in lung adenocarcinoma cells through the Wnt/β‑catenin pathway and possesses prognostic value in lung adenocarcinoma.RFC3 通过 Wnt/β-catenin 通路诱导肺腺癌细胞发生上皮-间充质转化，并且在肺腺癌中具有预后价值。
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