Sánchez Isabel Cristina, Segura Caro Juan Aicardo, Galeano Elkin, Alzate Fernando, Ossa-Giraldo Ana Claudia
Infettare Research Group, School of Medicine, Universidad Cooperativa de Colombia, Calle 50 No. 40-74, Medellín, Colombia.
Biociencias Research Group, Faculty of Health Sciences, Institución Universitaria Colegio Mayor de Antioquia, Carrera 78 #65-46, Medellín, Colombia.
Sci Rep. 2024 Dec 28;14(1):30643. doi: 10.1038/s41598-024-65961-x.
Bacterial resistance, a global public health concern prioritized by the World Health Organization, is particularly alarming in Staphylococcus aureus and Escherichia coli. Urgently addressing this, the search for new antibiotics has turned to plant essential oils. Our study focused essential oils derived from Colombian plants Croton killipianus, Croton smithianus, Croton leptostachyus, Croton hondensis, and Croton gossypiifolius. We performed antimicrobial susceptibility tests targeting Staphylococcus aureus, methicillin-resistant Staphylococus aureus, sensitive Escherichia coli, and ampicillin- and streptomycin-resistant Escherichia coli. Simultaneosly, citotoxic assays and chemical analysis were carried out. The essential oil derived from C. hondensis demonstrated superior inhibitory efficacy, effectively targeting methicillin-resistant S. aureus, susceptible S. aureus, and both sensitive and ampicillin- and streptomycin-resistant strains of E. coli. Furthermore, it exhibited notable potential for protective activity in Chinese hamster ovary cells. C. killipianus manifested inhibitory effects against MRSA and susceptible S. aureus, whereas C. smithianus specifically affected susceptible strains of S. aureus. Chemical analysis of the essential oils revealed rich content in phenolic compounds, flavonoids, tannins, and steroids. Gas-coupled mass spectrometry identified key compounds like γ-muurolene, α-humulene, (E)-caryophyllene, α-copaene, curcumene, and (E)-nerolidol. These findings underscore C. hondensis, C. killipianus, and C. smithianus as potential natural sources for antibacterial agent development.
细菌耐药性是世界卫生组织列为全球公共卫生重点关注的问题，在金黄色葡萄球菌和大肠杆菌中尤为令人担忧。为了紧急应对这一问题，寻找新型抗生素的工作已转向植物精油。我们的研究聚焦于源自哥伦比亚植物基氏巴豆、史密斯巴豆、细穗巴豆、洪都拉斯巴豆和棉叶巴豆的精油。我们针对金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌、敏感大肠杆菌以及耐氨苄青霉素和链霉素的大肠杆菌进行了药敏试验。同时，还进行了细胞毒性试验和化学分析。源自洪都拉斯巴豆的精油显示出卓越的抑制效果，能有效针对耐甲氧西林金黄色葡萄球菌、敏感金黄色葡萄球菌以及敏感和耐氨苄青霉素及链霉素的大肠杆菌菌株。此外，它在中国仓鼠卵巢细胞中展现出显著的保护活性潜力。基氏巴豆对耐甲氧西林金黄色葡萄球菌和敏感金黄色葡萄球菌表现出抑制作用，而史密斯巴豆则特别影响金黄色葡萄球菌的敏感菌株。对这些精油的化学分析表明，其富含酚类化合物、黄酮类、单宁和甾体。气相色谱 - 质谱联用仪鉴定出了关键化合物，如γ-依兰油烯、α-葎草烯、（E）-石竹烯、α-可巴烯、姜黄烯和（E）-橙花叔醇。这些发现强调了洪都拉斯巴豆、基氏巴豆和史密斯巴豆作为开发抗菌剂潜在天然来源的地位。
