Rocha Renan Rhonalty, Matos Maria Nágila Carneiro, Guerrero Jesús Alberto Pérez, Cavalcante Rafaela Mesquita Bastos, Melo Ramaiana Soares, Azevedo Águida Maria Albuquerque, Pereira Antônio Mateus Gomes, Lopes Pedro Henrique Ribeiro, Rodrigues Tigressa Helena Soares, Bandeira Paulo Nogueira, Gomes Geovany Amorim, Catunda Junior Francisco Eduardo Aragão, Carneiro-Torres Daniela Santos, Silva José Roberto Viana, Carneiro Victor Alves
Laboratory of Biofilms and Antimicrobial Agents (LaBAM), Federal University of Ceará, Sobral, 62048-280, Ceará, Brazil.
Center of Molecular Bioprospecting and Applied Experimentation (NUBEM), University Center INTA - UNINTA, Sobral, 62050-100, Ceará, Brazil.
Microb Pathog. 2021 Jul;156:104934. doi: 10.1016/j.micpath.2021.104934. Epub 2021 May 5.
The aim of this work was to evaluate the chemical composition and antibacterial activity of Croton tetradenius Baill. (CTEO) and C. pulegiodorus Baill. (CPEO) essential oils against Staphylococcus aureus, and their synergism with antibiotics. The essential oils (EOs) were extracted by hydrodistillation and chemically characterized by gas chromatography-mass spectrometry (CG-MS) and gas chromatography with flame ionization detection (CG-FID). The antimicrobial action of the EOs was tested against two standard strains and four clinical isolates of S. aureus using the disk-diffusion agar method and the microdilution assay. The bacterial kinetic growth was also determined. The synergistic effect between EOs and antimicrobials was analyzed by the checkerboard test. CTEO and CPEO yielded 0.47 and 0.37% w/w and the most common components were p-cymene (28.24%), camphor (17.76%) and α-phellandrene (8.98%), and trans-chrysanthenyl acetate (27.05%), α-terpinene (19.21%) and p-cymene (12.27%), respectively. The disk-diffusion test showed that the bacteria are sensitive to the agents tested. The MIC in the presence of the CTEO it was 4000 μg/mL, while for the CPEO it was 8000 μg/mL, except for clinical isolate 4B. The MBC for strains treated with CTEO were 8000 μg/mL, with the exception of isolates 8B and 0 A 4000 μg/mL. For the CPEO, all strains showed a concentration above 8000 μg/mL. The growth curve showed that CTEO and CPEO altered growth kinetics, delaying the lag phase and reducing the log phase. In combination with antibiotics, both essential oils showed synergisms effect with oxacillin and ampicillin, and additive effect with benzylpenicillin. CTEO and CPEO showed antibacterial action against S. aureus strains, showing as a promise natural alternative in clinical therapy.
本研究旨在评估四倍体巴豆（CTEO）和普列吉奥多斯巴豆（CPEO）精油的化学成分、对金黄色葡萄球菌的抗菌活性及其与抗生素的协同作用。通过水蒸馏法提取精油，并采用气相色谱 - 质谱联用（CG - MS）和带火焰离子化检测的气相色谱法（CG - FID）对其进行化学表征。使用纸片扩散琼脂法和微量稀释法测试了精油对两株标准菌株和四株金黄色葡萄球菌临床分离株的抗菌作用，并测定了细菌的生长动力学。通过棋盘法分析了精油与抗菌药物之间的协同作用。CTEO和CPEO的得率分别为0.47%和0.37%（w/w），最常见的成分分别为对伞花烃（28.24%）、樟脑（17.76%）和α - 水芹烯（8.98%），以及反式菊烯乙酸酯（27.05%）、α - 萜品烯（19.21%）和对伞花烃（12.27%）。纸片扩散试验表明，细菌对所测试的药剂敏感。在CTEO存在下，除临床分离株4B外，最低抑菌浓度（MIC）为4000μg/mL，而CPEO的MIC为8000μg/mL。用CTEO处理的菌株的最低杀菌浓度（MBC）除分离株8B和0A为4000μg/mL外，其余均为8000μg/mL。对于CPEO，所有菌株的浓度均高于8000μg/mL。生长曲线表明，CTEO和CPEO改变了生长动力学，延迟了延迟期并缩短了对数期。与抗生素联合使用时，两种精油与苯唑西林和氨苄西林均表现出协同作用，与苄青霉素表现出相加作用。CTEO和CPEO对金黄色葡萄球菌菌株具有抗菌作用，有望成为临床治疗中的天然替代物。
