Wang Fang, Wu Wanyu, He Xuejia, Qian Ping, Chang Jiahui, Lu Zhaoxu, Guo Jin, Bao Yihua, Guan Hongyan, Zhang Ting
Beijing Municipal Key Laboratory of Child Development and Nutriomics, Capital Institute of Pediatrics, 2 Yabao Road, Chaoyang District, Beijing, 100020, China.
Capital Institute of Pediatrics-Peking University Teaching Hospital, Beijing, China.
Sci Rep. 2024 Dec 28;14(1):31072. doi: 10.1038/s41598-024-82150-y.
Physical exercise is beneficial to keep physical and mental health. The molecular mechanisms underlying exercise are still worth exploring. The healthy adult mice after six weeks of moderate-intensity exercise (experimental group) and sedentary mice (control group) were used to perform transcriptomic, proteomic, lactylation modification, and metabolomics analysis. In addition, gene sets related to hypoxia, glycolysis, and fatty acid metabolism were used to aid in the screening of hub genes. The mMCP-counter was employed to evaluate infiltration of immune cells in murine liver tissues. Transcriptomics analysis revealed 82 intersection genes related to hypoxia, glycolysis, and fatty acid metabolism. Proteomics and lactylation modification analysis identified 577 proteins and 141 differentially lactylation modification proteins. By overlapping 82 intersection genes with 577 differentially expressed proteins and 141 differentially lactylation modification proteins, three hub genes (Aldoa, Acsl1, and Hadhb) were obtained. The immune infiltration analysis revealed a decreased score for monocytes/macrophages and an increased score for endothelial cells in the experimental group. Then, 459 metabolites in positive mode and 181 metabolites in negative mode were identified. The "Metabolic pathways" (mmu01100) was a common pathway between intersection genes-enriched pathways and metabolites-enriched pathways. These findings highlight the pivotal roles of hub genes in the glycolysis and fatty acid metabolism under the context of chronic exercise.
体育锻炼有益于保持身心健康。运动背后的分子机制仍值得探索。将六周中等强度运动后的健康成年小鼠（实验组）和久坐不动的小鼠（对照组）用于进行转录组学、蛋白质组学、乳酸化修饰和代谢组学分析。此外，利用与缺氧、糖酵解和脂肪酸代谢相关的基因集来辅助筛选核心基因。采用mMCP-counter评估小鼠肝脏组织中免疫细胞的浸润情况。转录组学分析揭示了82个与缺氧、糖酵解和脂肪酸代谢相关的交集基因。蛋白质组学和乳酸化修饰分析鉴定出577种蛋白质和141种差异乳酸化修饰蛋白质。通过将82个交集基因与577种差异表达蛋白质和141种差异乳酸化修饰蛋白质进行重叠，获得了三个核心基因（Aldoa、Acsl1和Hadhb）。免疫浸润分析显示，实验组中单核细胞/巨噬细胞的评分降低，内皮细胞的评分升高。然后，在正模式下鉴定出459种代谢物，在负模式下鉴定出181种代谢物。“代谢途径”（mmu01100）是交集基因富集途径和代谢物富集途径之间的共同途径。这些发现突出了核心基因在慢性运动背景下糖酵解和脂肪酸代谢中的关键作用。
