Liu Xia, Dong Shixiang, Ding Yu, Li Jinjin, Wang Jingyuan
Department of Reproductive Medicine, the Affiliated Hospital of Qingdao University, 59 Haier Road, Qingdao, 266000, China.
Department of Obstetrics and Gynecology, the Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, 266061, China.
J Assist Reprod Genet. 2025 Mar;42(3):809-815. doi: 10.1007/s10815-024-03359-4. Epub 2024 Dec 27.
In China, the prevalence of hepatitis B virus (HBV) infection among infertile couples is a significant clinical problem. It is necessary to determine the effect of HBV infection on embryo development.
The 4301 fresh cycles and 5763 frozen embryo transfer (FET) cycles were grouped according to the couple with or without HBV infection. The embryo fertilization rate, cleavage rate, transplantable embryo rate, and rate of high-quality embryos were analysed. The methylation status of maternal antigen that embryos require (MATER), zygote arrest 1 (ZAR1) and growth differentiation factor 9 (GDF9) genes in the peripheral blood of assisted reproductive technology (ART) women was detected by methylation-specific polymerase chain reaction (MSP).
The pregnancy rate of the female HBV-positive group was significantly lower than that of the HBV-negative group. The fertilization rate of intracytoplasmic sperm injection (ICSI) cycles in the male HBV-positive group was significantly lower than that of the male HBV-negative group. There were no differences in biochemistry or clinical pregnancy rates among the FET groups. The promoter methylation of GDF9 in HBV-positive ART women was higher than that in HBV-negative ART women, and that of ZAR1 in HBV-positive ART women was lower than that in HBV-negative ART women.
It was a detrimental effect of HBV infection on in vitro fertilization (IVF) and ICSI treatment outcomes in women. The HBV infection was associated with the maternal genes promoting methylation.
在中国，不孕夫妇中乙肝病毒（HBV）感染的患病率是一个重大的临床问题。确定HBV感染对胚胎发育的影响很有必要。
将4301个新鲜周期和5763个冻融胚胎移植（FET）周期按照夫妇是否感染HBV进行分组。分析胚胎受精率、卵裂率、可移植胚胎率和优质胚胎率。通过甲基化特异性聚合酶链反应（MSP）检测辅助生殖技术（ART）女性外周血中胚胎所需母体抗原（MATER）、合子阻滞1（ZAR1）和生长分化因子9（GDF9）基因的甲基化状态。
女性HBV阳性组的妊娠率显著低于HBV阴性组。男性HBV阳性组卵胞浆内单精子注射（ICSI）周期的受精率显著低于男性HBV阴性组。FET组之间的生化或临床妊娠率没有差异。HBV阳性ART女性中GDF9的启动子甲基化高于HBV阴性ART女性，而HBV阳性ART女性中ZAR1的启动子甲基化低于HBV阴性ART女性。
HBV感染对女性体外受精（IVF）和ICSI治疗结局有不利影响。HBV感染与母体基因甲基化促进有关。
