Saha Tisa Rani, Kang Nam Kyu, Lee Eun Yeol
Department of Chemical Engineering (BK21 FOUR Integrated Engineering), Kyung Hee University, Yongin-si, Gyeonggi-do, 17104, Republic of Korea.
Department of Chemical Engineering, College of Engineering, Kyung Hee University, Yongin- si, Gyeonggi-do, 17104, Republic of Korea.
J Biol Eng. 2024 Dec 27;18(1):73. doi: 10.1186/s13036-024-00473-w.
The biological production of lipids presents a sustainable method for generating fuels and chemicals. Recognized as safe and enhanced by advanced synthetic biology and metabolic engineering tools, yeasts are becoming versatile hosts for industrial applications. However, lipids accumulate predominantly as triacylglycerides in yeasts, which are suboptimal for industrial uses. Thus, there have been efforts to directly produce free fatty acids and their derivatives in yeast, such as fatty alcohols, fatty aldehydes, and fatty acid ethyl esters. This review offers a comprehensive overview of yeast metabolic engineering strategies to produce free fatty acids and their derivatives. This study also explores current challenges and future perspectives for sustainable industrial lipid production, particularly focusing on engineering strategies that enable yeast to utilize alternative carbon sources such as CO, methanol, and acetate, moving beyond traditional sugars. This review will guide further advancements in employing yeasts for environmentally friendly and economically viable lipid production technologies.
脂质的生物生产为生成燃料和化学品提供了一种可持续的方法。酵母被公认为安全且借助先进的合成生物学和代谢工程工具得到了强化，正成为工业应用中用途广泛的宿主。然而，脂质在酵母中主要以三酰甘油的形式积累，这对于工业用途而言并非最优。因此，人们一直在努力在酵母中直接生产游离脂肪酸及其衍生物，如脂肪醇、脂肪醛和脂肪酸乙酯。本综述全面概述了用于生产游离脂肪酸及其衍生物的酵母代谢工程策略。本研究还探讨了可持续工业脂质生产的当前挑战和未来前景，特别关注使酵母能够利用替代碳源（如一氧化碳、甲醇和乙酸盐）而非传统糖类的工程策略。本综述将指导在利用酵母实现环境友好且经济可行的脂质生产技术方面取得进一步进展。
