Faizi Fariba, Mahjub Reza, Torabi Negin, Motavallihaghi Seyedmousa, Fallah Mohammad
Department of Medical Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Faculty of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran.
Parasit Vectors. 2024 Dec 27;17(1):542. doi: 10.1186/s13071-024-06473-5.
Cystic echinococcosis (CE) is a common neglected parasitic disease. Nanoparticles containing drugs have been widely utilized in various formulations for several purposes, including improving the bioavailability of drugs by increasing the solubility and dissolution rate of the nanoparticles. The purpose of this study was to evaluate the effects of solid lipid nanoparticles containing albendazole and conjugated to albumin (B-SLN + ABZ) as a novel treatment approach for hydatid cysts in vivo.
Albendazole-loaded solid lipid nanoparticles were prepared by emulsification and solvent evaporation method. The experimental mice were assessed for prophylactic and therapeutic effects of the drugs. Ultrastructural changes were observed by transmission electron microscopy.
The variance analysis of the fitted model indicated that the Glyceryl monostearate (GMS)/soy lecithin concentration ratio and the amount of albendazole had a significant effect on nanoparticle size. The GMS/soy lecithin concentration ratio and the amount of albendazole had a notable effect on nanoparticle polydispersity index (PdI) and entrapment efficiency (EE%), respectively. During chemoprophylaxis, the B-SLN + ABZ group showed a lower number and weight of cysts (0.90 ± 0.73 and 15.01 ± 10.46, respectively) compared with the ABZ + SLN group (1.4 ± 0.51 and 26.73 ± 9.92, respectively). In addition, therapeutic efficacy analysis showed a significant reduction in wet weights of metacestodes in mice treated with both B-SLN + ABZ (29.37 ± 13.82 mg) and SLN + ABZ (35.88 ± 7.49 mg) compared with the control group (59.78 ± 3.80 mg).
The results showed that B-SLN + ABZ nanoparticles were more effective against E. granulosus cysts compared with free ABZ. The cysts in the animals receiving B-SLN + ABZ every 24 h showed more ultrastructural changes.
囊型包虫病（CE）是一种常见的被忽视的寄生虫病。含药纳米颗粒已被广泛应用于各种制剂中以实现多种目的，包括通过提高纳米颗粒的溶解度和溶解速率来提高药物的生物利用度。本研究的目的是评估载有阿苯达唑并与白蛋白偶联的固体脂质纳米颗粒（B-SLN + ABZ）作为一种新型治疗方法对体内包虫囊肿的影响。
采用乳化溶剂蒸发法制备载阿苯达唑的固体脂质纳米颗粒。对实验小鼠评估药物的预防和治疗效果。通过透射电子显微镜观察超微结构变化。
拟合模型的方差分析表明，单硬脂酸甘油酯（GMS）/大豆卵磷脂浓度比和阿苯达唑的量对纳米颗粒大小有显著影响。GMS/大豆卵磷脂浓度比和阿苯达唑的量分别对纳米颗粒多分散指数（PdI）和包封率（EE%）有显著影响。在化学预防期间，与阿苯达唑+固体脂质纳米颗粒组（分别为1.4±0.51和26.73±9.92）相比，B-SLN + ABZ组的囊肿数量和重量更低（分别为0.90±0.73和15.01±10.46）。此外，治疗效果分析表明，与对照组（59.78±3.80 mg）相比，用B-SLN + ABZ（29.37±13.82 mg）和固体脂质纳米颗粒+阿苯达唑（35.88±7.49 mg）治疗的小鼠中，原头蚴的湿重显著降低。
结果表明，与游离阿苯达唑相比，B-SLN + ABZ纳米颗粒对细粒棘球绦虫囊肿更有效。每24小时接受B-SLN + ABZ的动物体内的囊肿显示出更多的超微结构变化。
