Gong Lei, Liu Hai-Qing, Hua Ye, Zhang Ya-Yun, Hasan Md Mahadi
School of Agriculture and Bioengineering, Longdong University, Qingyang, China.
Gansu Key Laboratory of Protection and Utilization for Biological Resources and Ecological Restoration, Qingyang, China.
Plant Signal Behav. 2025 Dec;20(1):2446865. doi: 10.1080/15592324.2024.2446865. Epub 2024 Dec 28.
Abscisic acid (ABA) mediated stomatal closure is a highly effective mode of active stomatal regulation under drought stress. Previous studies on stomatal regulation have primarily focused on the leaves of vascular plants, while research on the stomatal behavior of bulbous plants remains unknown. In addition, ABA-induced stomatal regulation in bulbs has yet to be explored. Therefore, we aim to investigate the ABA-induced active regulation in the bulb of the Lanzhou lily ( var. unicolor). The morphological characteristics of epidermal strips were analyzed along with a stomatal aperture assay to investigate the bulb's stomatal response to ABA. Moreover, the mechanism of ABA signaling was explored using treatments with ABA signaling chemicals and corresponding scavengers. This study revealed that stomata are mainly distributed on the upper part and outer surface of the bulb. The guard cells of the lily bulb are inflated, and the stomata have a nearly circular shape with relatively low stomatal density. Exogenous ABA was found to induce varying degrees of stomatal closure in a dose-dependent manner, with significant stomatal aperture reduction observed after treatment with 10 µM ABA. Overall, the study indicated that both hydrogen peroxide (HO) and nitric oxide (NO) are involved in the ABA-induced stomatal closure process, with HO functioning as an upstream component of NO.
脱落酸（ABA）介导的气孔关闭是干旱胁迫下气孔主动调节的一种高效模式。以往关于气孔调节的研究主要集中在维管植物的叶片上，而关于球根植物气孔行为的研究仍不清楚。此外，ABA诱导的球根气孔调节尚未得到探索。因此，我们旨在研究ABA诱导的兰州百合（变种单色）球根中的主动调节。通过分析表皮条的形态特征以及进行气孔孔径测定，来研究球根对ABA的气孔反应。此外，使用ABA信号化学物质和相应清除剂进行处理，探索ABA信号传导机制。本研究表明，气孔主要分布在球根的上部和外表面。百合球根的保卫细胞膨胀，气孔呈近圆形，气孔密度相对较低。发现外源ABA以剂量依赖的方式诱导不同程度的气孔关闭，用10µM ABA处理后观察到气孔孔径显著减小。总体而言，该研究表明过氧化氢（HO）和一氧化氮（NO）都参与了ABA诱导的气孔关闭过程，其中HO作为NO的上游成分发挥作用。
