Li Jiaming, Su Jianfen, Li Minghui, Wu Yaofen, Chen Huiqiang, Fu Xihua, Yao Hongliang, Chen Jinping, Liu Yuntao, Zan Jie
School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, Guangdong, China.
The Affiliated Panyu Central Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
Mikrochim Acta. 2024 Dec 28;192(1):39. doi: 10.1007/s00604-024-06903-2.
Time-resolved fluorescence immunochromatographic test strips (TRFIS) was developed for the rapid detection of hepatocellular carcinoma (HCC)-specific plasma exosomes (hExos) by targeting the hExo-surface membrane protein glypican-3 (GPC3). The GPC3-TRFIS could directly detect plasma exosomes without the isolation and purification process, and the whole immunoassay could be completed within 15 min. The visual detection limit of GPC3-TRFIS was 3.44 × 10^9 particles/mL, with a minimum detection limit of 1.8 × 10^9 particles/mL. For analysis of the clinical HCC samples, GPC3-TRFIS shows high specificity for detection of hExo, and was nearly unreactive for healthy donors' samples. GPC3-TRFIS was able to efficiently distinguish HCC patients (19 cases) from healthy donors (19 cases). Overall, the developed TRFIS offers the benefits of high sensitivity, simple operation, and no need of large precision instruments and professional technical personnel for rapid detection of plasma hExos, and supplies a novel approach for early screening of HCC.
时间分辨荧光免疫层析试纸条（TRFIS）被开发用于通过靶向肝细胞癌（HCC）特异性血浆外泌体（hExos）表面膜蛋白磷脂酰肌醇蛋白聚糖-3（GPC3）来快速检测HCC。GPC3-TRFIS无需分离和纯化过程即可直接检测血浆外泌体，整个免疫测定可在15分钟内完成。GPC3-TRFIS的视觉检测限为3.44×10^9颗粒/毫升，最低检测限为1.8×10^9颗粒/毫升。对于临床HCC样本分析，GPC3-TRFIS对hExo检测具有高特异性，对健康供体样本几乎无反应。GPC3-TRFIS能够有效区分HCC患者（19例）和健康供体（19例）。总体而言，所开发的TRFIS具有高灵敏度、操作简单、无需大型精密仪器和专业技术人员即可快速检测血浆hExos的优点，并为HCC的早期筛查提供了一种新方法。
