Brown Phillip J, Green J Edward F, Binder Benjamin J, Osborne James M
School of Computer and Mathematical Sciences, University of Adelaide, Adelaide, Australia.
School of Mathematics and Statistics, University of Melbourne, Melbourne, Australia.
Math Biosci. 2025 Feb;380:109367. doi: 10.1016/j.mbs.2024.109367. Epub 2024 Dec 26.
A model using the rigid body multi-cellular framework (RBMCF) is implemented to investigate the mechanisms of buckling of an epithelial monolayer. Specifically, the deformation of a monolayer of epithelial cells which are attached to a basement membrane and the surrounding stromal tissue. The epithelial monolayer, supporting basement membrane and stromal tissue are modelled using two separate vertex dynamics models (one for the epithelial monolayer layer and one for the basement membrane and stromal tissue combined) and interactions between the two are considered using the RBMCF to ensure biologically realistic interactions. Model simulations are used to investigate the effects of cell-stromal attachment and membrane rigidity on buckling behaviour. We demonstrate that there are two competing modes of buckling, stromal deformation and stromal separation.
采用刚体多细胞框架（RBMCF）的模型被用于研究上皮单层细胞屈曲的机制。具体而言，研究附着于基底膜和周围基质组织的上皮细胞单层的变形情况。上皮单层细胞、支撑基底膜和基质组织分别使用两个独立的顶点动力学模型进行建模（一个用于上皮单层细胞，另一个用于基底膜和基质组织的组合），并使用RBMCF考虑两者之间的相互作用，以确保生物学上的真实相互作用。通过模型模拟来研究细胞与基质附着以及膜刚度对屈曲行为的影响。我们证明存在两种相互竞争的屈曲模式，即基质变形和基质分离。
