Goujani Shayan Momeni, Koopaie Maryam, Safarian Fateme Hamta, Hakimiha Neda, Younespour Shima
Department of Oral Medicine, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Department of Oral Medicine, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Photodiagnosis Photodyn Ther. 2025 Feb;51:104457. doi: 10.1016/j.pdpdt.2024.104457. Epub 2024 Dec 27.
Squamous cell carcinoma (SCC) is the most common malignancy of the head and neck region. Combination therapy potentially enhances the effectiveness beyond that of each treatment alone. This study aimed to assess whether photodynamic therapy (PDT), using methylene blue as a photosensitizer in conjunction with doxorubicin, produces synergistic effects on the apoptosis of the oral squamous cell carcinoma (OSCC) cell line.
The human oral epidermal carcinoma cell line (KB cell line, NCBI Code: C152) was cultured in Dulbecco's modified Eagle's medium. Following at least 24 hours of incubation, the OSCC cells were distributed into six groups, with groups 1-3 and 5 performed in the dark to prevent any light interference. 1: control group; 2: treated with 3.2 μg/mL methylene blue; 3: exposed to various concentrations of doxorubicin; 4: PDT group (methylene blue + 660 nm light); 5: treated with both doxorubicin and methylene blue; and finally, 6: treated with PDT (methylene blue + 660 nm light) in conjunction with doxorubicin. Flow cytometry methods were used to assess apoptosis. Analysis of variance (ANOVA) was used to compare quantitative variables between groups, and Tukey's test was applied for pairwise group comparisons.
Flow cytometry analysis revealed that the highest level of cellular apoptosis occurred in the group treated with PDT in conjunction with doxorubicin.
PDT using the photosensitizer methylene blue, in combination with doxorubicin, can serve as an effective agent for inducing apoptosis in OSCC cells.
鳞状细胞癌（SCC）是头颈部最常见的恶性肿瘤。联合治疗可能比单独使用每种治疗方法更有效。本研究旨在评估使用亚甲蓝作为光敏剂并联合阿霉素的光动力疗法（PDT）是否对口腔鳞状细胞癌（OSCC）细胞系的凋亡产生协同作用。
人口腔表皮癌细胞系（KB细胞系，NCBI代码：C152）在杜氏改良 Eagle 培养基中培养。孵育至少24小时后，将OSCC细胞分为六组，其中第1 - 3组和第5组在黑暗中进行以防止任何光干扰。1：对照组；2：用3.2μg/mL亚甲蓝处理；3：暴露于不同浓度的阿霉素；4：PDT组（亚甲蓝 + 660nm光）；5：用阿霉素和亚甲蓝联合处理；最后，6：用PDT（亚甲蓝 + 660nm光）联合阿霉素处理。采用流式细胞术方法评估细胞凋亡。方差分析（ANOVA）用于比较组间定量变量，Tukey检验用于组间两两比较。
流式细胞术分析显示，联合使用PDT和阿霉素处理的组中细胞凋亡水平最高。
使用光敏剂亚甲蓝联合阿霉素的PDT可作为诱导OSCC细胞凋亡的有效药物。
