Yin Shuyan, Wang Yi-Xuan, Hou Cheng, Wang Jing, Xu Jing, Jiang Xinbai, Chen Dan, Mu Yang, Shen Jinyou
Key Laboratory of Environmental Remediation and Ecological Health, Ministry of Industry and Information Technology, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu, 210094, China; Engineering Research Centre of Chemical Pollution Control, Ministry of Education, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu, 210094, China.
Key Laboratory of Environmental Remediation and Ecological Health, Ministry of Industry and Information Technology, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu, 210094, China.
Bioresour Technol. 2025 Mar;419:132020. doi: 10.1016/j.biortech.2024.132020. Epub 2024 Dec 26.
Anammox coupled partial S-driven autotrophic denitrification (PSAD) technology represents an innovative approach for removing nitrogen from wastewater. The research highlighted the crucial role of biofilm on sulfur particles in the nitrogen removal process. Further analysis revealed that sulfur-oxidizing bacteria (SOB) are primarily distributed in the inner layer of the biofilm, while anammox bacteria (AnAOB) are relatively evenly distributed in inner and outer layers, with Thiobacillus and Candidatus Brocadia being the dominant species, respectively. Except for anammox and PSAD processes, N isotope labeling tests determined that sulfur reshaped nitrogen metabolism pathways, providing solid evidence for the occurrence of sulfammox process. SOB and AnAOB collaborate in nitrogen and sulfur conversion, with SOB-drived PSAD processes reducing nitrate to nitrite for AnAOB to remove ammonia. Conversely, the nitrate produced from anammox process can be reused by SOB. Metagenomic analyses verified that SOB drove the PSAD process through encoding soxBYZ gene, while AnAOB might play an important role in simultaneously driving the anammox and sulfammox processes. These findings underscore the importance of biofilm and clarify the nitrogen-sulfur cycle mechanisms within the coupled system.
厌氧氨氧化耦合部分硫驱动自养反硝化（PSAD）技术是一种去除废水中氮的创新方法。该研究强调了生物膜在硫颗粒上对脱氮过程的关键作用。进一步分析表明，硫氧化细菌（SOB）主要分布在生物膜的内层，而厌氧氨氧化细菌（AnAOB）相对均匀地分布在内层和外层，其中硫杆菌属和“Candidatus Brocadia”分别为优势菌种。除了厌氧氨氧化和PSAD过程外，氮同位素标记试验确定硫重塑了氮代谢途径，为硫氨氧化过程的发生提供了确凿证据。SOB和AnAOB在氮和硫的转化中相互协作，SOB驱动的PSAD过程将硝酸盐还原为亚硝酸盐供AnAOB去除氨。相反，厌氧氨氧化过程产生的硝酸盐可被SOB再利用。宏基因组分析证实，SOB通过编码soxBYZ基因驱动PSAD过程，而AnAOB可能在同时驱动厌氧氨氧化和硫氨氧化过程中发挥重要作用。这些发现强调了生物膜的重要性，并阐明了耦合系统内的氮硫循环机制。
