参与可育和乏情荷斯坦奶牛繁殖性状的NOTCH2基因非翻译区的四个单核苷酸多态性。

Four SNPs in the untranslated region of the NOTCH2 gene involved in reproductive traits in fertile and anestrum Holstein Friesian cattle.

作者信息

Mahmoud Karima Gh M, Sosa Ahmed S A, Darwish Hassan R H, Ibrahim Sally, Hasanain Mahmoud H, Almadaly Essam A, Sakr Abdelaziz M, Shams A Sh E, Ilie Daniela Elena

机构信息

Department of Animal Reproduction& AI, National Research Centre, Tahrir Street, Dokki, Giza, 12622, Egypt.

Cell Biology Department and A.I, Institute of Biotechnology Research, National Research Centre, Dokki, 12622, Giza, Egypt.

出版信息

BMC Vet Res. 2024 Dec 28;20(1):581. doi: 10.1186/s12917-024-04393-1.

DOI:10.1186/s12917-024-04393-1

PMID:39732698

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11681750/

Abstract

BACKGROUND

There are no studies belong NOTCH2 gene polymorphism in relation to reproductive and productive traits in Holstein cattle. The objective of the present study was to investigate the effect of NOTCH2 gene polymorphisms on productive and reproductive performance of fertile and anestrum cattle.

METHODS

The cattle were classified into anestrus for 3-12 months postpartum (n = 115, 37.7%) and normal cyclic fertile (n = 190, 62.3%). Age at 1st calving, calving interval, calf birth weight, milk yield and days of milking data were obtained from the farm records for all animals. Blood samples were collected from fertile and anestrum animals for DNA extraction and the PCR as well as single-strand conformation polymorphism techniques (SSCP) were performed for detection of the NOTCH2 gene polymorphism.

RESULTS

The sequencing results showed that NOTCH2 gene in cattle is polymorphic with 5 genotypes AA, AG, GG, CC and CT. Four novel SNPs as transition single base substitution mutations were detected at 3: 23,192,033 (A/G), 3:23192149 (C/T), 3:23192071 (C/T) and 3:23192143 (G/A). GG genotype of NOTCH2 gene was associated with shorter calving interval and heavier calf birth weight in both fertile and anestrum cattle.

CONCLUSIONS

NOTCH2 gene in cattle could be used as a genetic marker involved in reproductive traits in both fertile and anestrum cattle.

摘要

背景

尚无关于荷斯坦奶牛NOTCH2基因多态性与繁殖和生产性状关系的研究。本研究的目的是调查NOTCH2基因多态性对可育和处于乏情期奶牛生产性能和繁殖性能的影响。

方法

将奶牛分为产后3至12个月处于乏情期的（n = 115，37.7%）和正常周期性可育的（n = 190，62.3%）。从农场记录中获取所有动物的首次产犊年龄、产犊间隔、犊牛出生体重、产奶量和挤奶天数数据。从可育和处于乏情期的动物采集血样用于DNA提取，并进行PCR以及单链构象多态性技术（SSCP）以检测NOTCH2基因多态性。

结果

测序结果显示，奶牛的NOTCH2基因具有多态性，有AA、AG、GG、CC和CT 5种基因型。在3个位置检测到4个新的单核苷酸多态性，为转换型单碱基替代突变：3: 23,192,033（A/G）、3:23192149（C/T）、3:23192071（C/T）和3:23192143（G/A）。NOTCH2基因的GG基因型与可育和处于乏情期奶牛较短的产犊间隔和较重的犊牛出生体重相关。