Priel E, Mittelman B, Efraim L, Priel T, Szaingurten-Solodkin I, Har-Vardi I
Department of Mechanical Engineering, Shamoon College of Engineering, Beer-Sheva, 84100, Israel.
NRCN-Materials Engineering Department, Beer-Sheva, 84190, Israel.
Sci Rep. 2024 Dec 28;14(1):31411. doi: 10.1038/s41598-024-83042-x.
It has long been speculated that the mechanical properties of the human oocyte can be an indicator for oocyte viability. Recent studies have demonstrated that embryo implantation rates, following Intra-Cytoplasmic Sperm Injection (ICSI) procedures, may be increased if the shear modulus value of the oocyte Zona Pellucida (ZP) is taken into consideration during embryo transfer. The shear modulus was determined by an iterative oocyte specific finite element (FE) analysis based on the clinical ICSI data. Nevertheless, the results obtained from the computational analysis may depend on the choice of the constitutive model used for the ZP. In the current study, three different hyper-elastic strain energy density functions (SEDF's) are considered. The Neo-Hookean (NH), Mooney-Rivlin (MR) and Ogden models (OG) were used to determine the ZP shear modulus from ICSI clinical data. The sensitivity of each SEDF in extracting the oocyte specific shear modulus is examined, for the first time. It is demonstrated that the NH and MR models are more sensitive than the OG model for determining the ZP shear modulus from clinical ICSI data. It is also demonstrated that the accuracy of ZP shear modulus identification process greatly depends on the value of suction pressure applied during the ICSI procedure.
长期以来，人们一直推测人类卵母细胞的力学特性可能是卵母细胞活力的一个指标。最近的研究表明，在胚胎移植过程中，如果考虑卵母细胞透明带（ZP）的剪切模量值，胞浆内单精子注射（ICSI）程序后的胚胎着床率可能会提高。剪切模量是根据临床ICSI数据通过迭代的卵母细胞特定有限元（FE）分析确定的。然而，从计算分析中获得的结果可能取决于用于ZP的本构模型的选择。在当前的研究中，考虑了三种不同的超弹性应变能密度函数（SEDF）。使用新胡克（NH）、穆尼-里夫林（MR）和奥格登模型（OG）从ICSI临床数据中确定ZP剪切模量。首次研究了每种SEDF在提取卵母细胞特定剪切模量方面的敏感性。结果表明，在从临床ICSI数据确定ZP剪切模量方面，NH和MR模型比OG模型更敏感。还表明，ZP剪切模量识别过程的准确性在很大程度上取决于ICSI程序中施加的抽吸压力值。
