Huang Ludi, Wang Xincen, Zhou Wei, Li Zeqi, Chen Chuanjing, Sun Yongye
School of Public Health, Qingdao University, Qingdao 266071, China.
Radiology Department of Qingdao Hospital, University of Health and Rehabilitation Sciences (Qingdao Municipal Hospital), China.
Int J Biol Macromol. 2025 Mar;292:139223. doi: 10.1016/j.ijbiomac.2024.139223. Epub 2024 Dec 27.
Osteoporosis is a systemic, progressive bone disease that causes metabolic disorders. Previous study identified the preventive effects of hydrolyzed egg yolk peptide (YPEP) on osteoporosis. However, the underlying antiosteoporosis mechanism remains unclear. Herein, 30 female rats were randomly divided into 5 groups (n = 6), including the sham, OVX, E2 (25 μg/kg/d 17β-estradiol), LYPEP (10 mg/kg/d YPEP), and HYPEP (40 mg/kg/d YPEP) groups. YPEP treatment significantly changed bone turnover marker levels and prevented the deterioration of bone structure and strength caused by ovariectomy. YPEP supplementation significantly changed endogenous metabolites related to lipid metabolism in the serum of ovariectomized rats, identifying 46 metabolites closely linked to bone biomarkers. Additionally, YPEP reduced the expression of the lipid metabolism-related protein peroxisome proliferator-activated receptor PPARγ and increased the expression of bone formation proteins BMP2 and RUNX2. Collectively, these results elucidated that YPEP improves osteoporosis by inhibiting lipogenesis to promote bone formation. This study provides novel evidence for the use of YPEP in treating osteoporosis.
骨质疏松症是一种导致代谢紊乱的全身性、进行性骨病。先前的研究确定了水解蛋黄肽（YPEP）对骨质疏松症的预防作用。然而，其潜在的抗骨质疏松机制仍不清楚。在此，将30只雌性大鼠随机分为5组（n = 6），包括假手术组、去卵巢组、E2组（17β-雌二醇25μg/kg/d）、LYPEP组（YPEP 10mg/kg/d）和HYPEP组（YPEP 40mg/kg/d）。YPEP治疗显著改变了骨转换标志物水平，并预防了去卵巢引起的骨结构和强度恶化。补充YPEP显著改变了去卵巢大鼠血清中与脂质代谢相关的内源性代谢物，鉴定出46种与骨生物标志物密切相关的代谢物。此外，YPEP降低了脂质代谢相关蛋白过氧化物酶体增殖物激活受体PPARγ的表达，并增加了骨形成蛋白BMP2和RUNX2的表达。总体而言，这些结果表明YPEP通过抑制脂肪生成来促进骨形成，从而改善骨质疏松症。本研究为YPEP用于治疗骨质疏松症提供了新的证据。
