钛盘表面涂覆的聚丙烯腈负载纳米姜黄素对成骨细胞反应的评估：体外细胞培养实验研究
Evaluation of osteoblast response to polyacrylonitrile infused nano-curcumin coated on titanium discs: Invitro study cell culture experimental study.
作者信息
Gupta Shilpi, Chander N Gopi, Bhatt Aravind, Anitha K V
机构信息
Department of Prosthodontics, SRM Dental College, Bharathi Salai, Ramapuram, Chennai, 89, India.
出版信息
J Oral Biol Craniofac Res. 2025 Jan-Feb;15(1):57-62. doi: 10.1016/j.jobcr.2024.12.003. Epub 2024 Dec 9.
PURPOSE
The study evaluated the influence of titanium discs, coated with polyacrylonitrile infused curcumin nanofibers on osteoblast activity.
MATERIALS AND METHODS
The titanium discs were coated with polyacrylonitrile nanofibers infused with curcumin. MG-63 cell lines were utilized for cell culture to assess osteoblast morphology upon exposure of curcumin on titanium discs. SEM comparison was made. Lactate Dehydrogenase (LDH) activity was measured after 2 and 7 days and the Alkaline Phosphatase (ALP) activity of the cells was quantified.
RESULTS
The results indicated that the coating had a notable impact on mineralization, LDH and ALP activities. Significant differences were observed between uncoated and coated samples. The SEM analysis indicated that curcumin enhanced bone growth when the Ti discs coated with curcumin are implanted in the bone.
CONCLUSION
Polyacrylonitrile infused nano-curcumin fibers coated on titanium discs potentially enhanced osteoblast response and mineralization.
目的
本研究评估了涂覆有注入姜黄素的聚丙烯腈纳米纤维的钛盘对成骨细胞活性的影响。
材料与方法
将钛盘涂覆注入姜黄素的聚丙烯腈纳米纤维。利用MG-63细胞系进行细胞培养，以评估姜黄素作用于钛盘时成骨细胞的形态。进行扫描电子显微镜（SEM）比较。在第2天和第7天后测量乳酸脱氢酶（LDH）活性，并对细胞的碱性磷酸酶（ALP）活性进行定量分析。
结果
结果表明，该涂层对矿化、LDH和ALP活性有显著影响。未涂层和涂层样品之间观察到显著差异。SEM分析表明，当将涂覆姜黄素的钛盘植入骨中时，姜黄素可促进骨生长。
结论
涂覆在钛盘上的注入纳米姜黄素的聚丙烯腈纤维可能增强成骨细胞反应和矿化作用。
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