Kong Linghui, Li Xinyu, Liu Taiyu, Yao Qingshou, Qin Jiayang
School of Pharmacy, Binzhou Medical University, Yantai, China.
Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, China.
Front Microbiol. 2024 Dec 13;15:1492179. doi: 10.3389/fmicb.2024.1492179. eCollection 2024.
Nicotinamide mononucleotide (NMN), one of the crucial precursors of nicotinamide adenine dinucleotide, has garnered considerable interest for its pharmacological and anti-aging effects, conferring potential health and economic benefits for humans. Lactic acid bacteria (LAB) are one of the most important probiotics, which is commonly used in the dairy industry. Due to its probiotic properties, it presents an attractive platform for food-grade NMN production. LAB have also been extensively utilized to enhance the functional properties of pharmaceuticals and cosmetics, making them promising candidates for large-scale up synthesis of NMN. This review provides an in-depth analysis of various metabolic engineering strategies, including enzyme optimization, pathway rewiring, and fermentation process enhancements, to increase NMN yields in LAB. It explores both CRISPR/Cas9 and traditional methods to manipulate key biosynthetic pathways. In particular, this study discussed future research directions, emphasizing the application of synthetic biology, systems biology, and AI-driven optimization to further enhance NMN production. It provides invaluable insights into developing scalable and industrially relevant processes for NMN production to meet the growing market demand.
烟酰胺单核苷酸（NMN）是烟酰胺腺嘌呤二核苷酸的关键前体之一，因其药理作用和抗衰老作用而备受关注，对人类具有潜在的健康和经济效益。乳酸菌（LAB）是最重要的益生菌之一，常用于乳制品行业。由于其益生菌特性，它为食品级NMN的生产提供了一个有吸引力的平台。LAB还被广泛用于增强药物和化妆品的功能特性，使其成为大规模合成NMN的有前途的候选者。本综述深入分析了各种代谢工程策略，包括酶优化、途径重新布线和发酵过程改进，以提高LAB中NMN的产量。它探讨了CRISPR/Cas9和传统方法来操纵关键生物合成途径。特别是，本研究讨论了未来的研究方向，强调合成生物学、系统生物学和人工智能驱动的优化在进一步提高NMN产量方面的应用。它为开发可扩展的、与工业相关的NMN生产工艺以满足不断增长的市场需求提供了宝贵的见解。
