Henry F J, Bassett J R
J Endocrinol. 1985 Mar;104(3):381-6. doi: 10.1677/joe.0.1040381.
Incubation of homogenized rat adrenal glands with sulphatase for 18 h resulted in a significant increase in the amount of unconjugated corticosterone that could be extracted from the gland and which could not be attributed to de-novo synthesis of corticosterone during the incubation. Therefore corticosterone may be stored within the adrenal gland as a sulphate conjugate rather than as unconjugated hormone. Exposure of animals to footshock stress resulted in an increase in the amount of unconjugated corticosterone in the gland and a disappearance of the conjugate form. The rapid disappearance of the sulphate conjugate may reflect the activation of a steroid sulphatase by ACTH. However, the release of corticosterone from a storage form such as corticosterone sulphate could not explain entirely the initial increase in plasma corticosterone concentration following the stress. It was calculated that a pair of adrenal glands would have to store up to 20 nmole corticosterone in order to account for the initial increase in plasma corticosterone. This level was much greater than that extracted from a pair of glands (4.8 nmol) even after sulphatase pretreatment.
将大鼠肾上腺匀浆与硫酸酯酶一起孵育18小时，导致可从腺体中提取的未结合皮质酮量显著增加，且这不能归因于孵育期间皮质酮的从头合成。因此，皮质酮可能以硫酸酯共轭物的形式而非未结合激素的形式储存在肾上腺中。使动物遭受足部电击应激会导致腺体中未结合皮质酮量增加，且共轭物形式消失。硫酸酯共轭物的快速消失可能反映了促肾上腺皮质激素（ACTH）对类固醇硫酸酯酶的激活作用。然而，从诸如硫酸皮质酮这样的储存形式中释放皮质酮并不能完全解释应激后血浆皮质酮浓度的初始升高。据计算，一对肾上腺必须储存多达20纳摩尔的皮质酮才能解释血浆皮质酮的初始升高。即使经过硫酸酯酶预处理，这个水平也远高于从一对腺体中提取的量（4.8纳摩尔）。
