Mohamed Heba R, Hamed Manal M, El-Wakil Eman A, Okasha Hend
Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Kornish El-Nile, Warrak El-Hadar, Giza 12411, Egypt.
Department of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Kornish El-Nile, Warrak El-Hadar, Giza 12411, Egypt.
Toxicol Rep. 2024 Nov 28;13:101829. doi: 10.1016/j.toxrep.2024.101829. eCollection 2024 Dec.
Due to their variability and safety, widespread research on phytochemicals continually encourages researchers to study various plants for their potential health benefits.
This study aims to explore the phytochemical constituents of the aerial parts of three spp.; , and existed in Egyptian nature using GC-MS analysis and studying their different biological activities in correlation to computational analysis.
Investigation of in vitro anti-inflammatory and anticancer activities and in silico studies of identified major compounds on VEGFR. Results: Thirty-three compounds were identified, octadecanoic acid, 2, 3-dihydroxypropyl ester, and 1H-Indene, 1-hexadecyl-2, 3-dihydro are the common compounds in the three extracts with different percentages. is the most extract with diverse phytoconstituents showing significant anticancer properties against the CACO2 cells with IC value equal to 31.78 µg/mL. Nevertheless, all extracts showed potent anti-inflammatory activity at high concentrations (500 µg/mL). , , and had IC on HepG2 cells equal to 73.64, 88.18, and 87.82 µg/mL. Molecular findings showed the three extracts had distinct effects on apoptosis modulation in HepG2 cells. Conclusion The findings suggest that the studied extracts had potential therapeutic properties as anti-inflammatory and anticancer agents, supported by an in-silico interaction study.
由于植物化学物质的多样性和安全性，对其进行的广泛研究不断促使研究人员研究各种植物以探寻其潜在的健康益处。
本研究旨在利用气相色谱 - 质谱联用（GC - MS）分析探索埃及自然生长的三种[植物名称未给出]地上部分的植物化学成分，并结合计算分析研究它们不同的生物活性。
研究体外抗炎和抗癌活性，并对鉴定出的主要化合物进行血管内皮生长因子受体（VEGFR）的计算机模拟研究。结果：鉴定出33种化合物，十八烷酸2,3 - 二羟丙酯和1 - 十六烷基 - 2,3 - 二氢 - 1H - 茚是三种提取物中的常见化合物，含量不同。[提取物名称未给出]是植物成分最多样化的提取物，对Caco2细胞显示出显著的抗癌特性，IC值等于31.78μg/mL。然而，所有提取物在高浓度（500μg/mL）时均显示出强大的抗炎活性。[提取物名称未给出]、[提取物名称未给出]和[提取物名称未给出]对HepG2细胞的IC值分别等于73.64、88.18和87.82μg/mL。分子研究结果表明，这三种提取物对HepG2细胞的凋亡调节有不同影响。结论：研究结果表明，这些提取物具有作为抗炎和抗癌药物的潜在治疗特性，计算机模拟相互作用研究对此提供了支持。
