Allmang C, Hussar P, Dūrītis I, Järveots T
Chair of Veterinary Biomedicine and Food Hygiene, Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Kreutzwaldi Str.62, Tartu 51006, Estonia.
Department of Anatomy, Institute of Biomedicine and Translational Medicine, Faculty of Medicine, University of Tartu, Ravila 19, Tartu 50411, Estonia.
Pol J Vet Sci. 2024 Sep;27(3):323-328. doi: 10.24425/pjvs.2024.149363.
In homeostasis, which plays an important role in the proper functioning and maintenance of the internal functioning of the body, kidneys play a key role in being responsible for the proper homeostasis of glucose. Among glucose transporters, sodium-dependent glucose co-transporters (SGLTs) have a major role in the kidney's ability to reabsorb glucose. Although the localization of these transporters has been extensively studied in mammals, there are still gaps in knowledge of the localization of SGLTs in birds of different age groups. The aim of this study was to immunolocalize in kidneys of hen chickens of different ages the sodium-dependent glucose co-transporters SGLT1 and SGLT2, comparing the localization between different age groups. The kidneys derived from 32 hen chickens (Gallus gallus domesticus) were devided equally into four age groups: 3, 7, 14, and 20 day old broilers, 8 indivuals in each group. The polyclonal primary antibodies Rabbit anti- SGLT1 and Rabbit anti-SGLT2 (Abcam, UK) were used together with the corresponding IHC kit (Abcam, UK). The results were visualized photographically using an AxioCam HRc camera (Germany) connected to a Zeiss Axioplan-2 Imaging microscope (Germany). The study revealed similar immunolocalization of SGLT1 and SGLT2 in the apical part of cells of proximal renal tubules in hen chickens' kidneys in all age groups. Strong staining of SGLT2 was noted also in the cytoplasm of epithelial cells of the proximal straight and convoluted tubules. Based on our study, the kidney tissue of newly hatched chickens is ready immediately after hatching for glucose reabsorption and transport, similarily to that of three-week-old chicks.
在对身体内部功能的正常运作和维持起着重要作用的内环境稳态中，肾脏在负责葡萄糖的适当内环境稳态方面发挥着关键作用。在葡萄糖转运蛋白中，钠依赖性葡萄糖协同转运蛋白（SGLTs）在肾脏重吸收葡萄糖的能力中起主要作用。尽管这些转运蛋白的定位在哺乳动物中已得到广泛研究，但不同年龄组鸟类中SGLTs定位的知识仍存在空白。本研究的目的是在不同年龄的母鸡肾脏中对钠依赖性葡萄糖协同转运蛋白SGLT1和SGLT2进行免疫定位，比较不同年龄组之间的定位情况。从32只母鸡（家鸡）获取的肾脏被平均分为四个年龄组：3日龄、7日龄、14日龄和20日龄的肉鸡，每组8只。使用兔抗SGLT1和兔抗SGLT2多克隆一抗（英国Abcam公司）以及相应的免疫组化试剂盒（英国Abcam公司）。使用连接到蔡司Axioplan - 2成像显微镜（德国）的AxioCam HRc相机（德国）对结果进行拍照可视化。研究显示，在所有年龄组的母鸡肾脏近端肾小管细胞顶端部分，SGLT1和SGLT2的免疫定位相似。在近端直小管和近端曲小管上皮细胞的细胞质中也观察到SGLT2的强染色。基于我们的研究，新孵化雏鸡的肾脏组织在孵化后立即就具备了葡萄糖重吸收和转运的能力，这与三周龄雏鸡的情况类似。
