Allmang Cristin, Hussar Piret, Dūrītis Ilmārs, Popovska-Percinic Florina
Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, 51006 Tartu, Estonia.
Faculty of Medicine, University of Tartu, 50411 Tartu, Estonia.
Curr Issues Mol Biol. 2024 Dec 18;46(12):14259-14269. doi: 10.3390/cimb46120854.
The kidney plays an essential role in the proper homeostasis of glucose. In the kidney, glucose transport is carried out across cell membranes by two families of glucose transporters-facilitated diffusion glucose transporters (GLUTs) and Na(+)-dependent glucose co-transporters (SGLT family). Among the transporters, sodium-dependent glucose co-transporters play a major role in the kidney's ability to reabsorb glucose. Although the localization of glucose transporters has been extensively studied in mammals, there are still knowledge gaps regarding the localization of SGLTs in birds. The aim of this research was to conduct a comparative study of the immunolocalization of the sodium-dependent glucose co-transporters SGLT1 and SGLT2 in the kidneys of healthy and T-2-mycotoxicated chickens. Immunohistochemical staining was carried out using the polyclonal primary antibodies SGLT1 and SGLT2 (Abcam, UK) in kidney tissue derived from seven healthy and seven T-2-mycotoxicated 7-day-old female layer-type Ross chickens (). The sections were stained using an immunohistochemistry kit (Abcam, UK). In the kidneys of the healthy birds, strong staining of SGLT1 and SGLT2 was observed in the cytoplasm of the epithelial cells of the proximal straight and convoluted tubules. In the kidneys of the birds of the T-2 toxin group, weak expression of SGLT1 and SGLT2 with morphological changes occurred, indicating reduced glucose transport in the urinary system during T-2 mycotoxicosis.
肾脏在葡萄糖的正常体内平衡中起着至关重要的作用。在肾脏中，葡萄糖通过两类葡萄糖转运蛋白跨细胞膜进行转运，即易化扩散葡萄糖转运蛋白（GLUTs）和钠依赖性葡萄糖共转运蛋白（SGLT家族）。在这些转运蛋白中，钠依赖性葡萄糖共转运蛋白在肾脏重吸收葡萄糖的能力中起主要作用。尽管在哺乳动物中对葡萄糖转运蛋白的定位已进行了广泛研究，但关于鸟类中SGLTs的定位仍存在知识空白。本研究的目的是对健康和T-2霉菌毒素中毒的鸡肾脏中钠依赖性葡萄糖共转运蛋白SGLT1和SGLT2的免疫定位进行比较研究。使用多克隆一抗SGLT1和SGLT2（英国Abcam公司）对7只健康和7只T-2霉菌毒素中毒的7日龄雌性罗斯蛋鸡的肾脏组织进行免疫组织化学染色。切片使用免疫组织化学试剂盒（英国Abcam公司）进行染色。在健康鸡的肾脏中，在近端直小管和近端曲小管上皮细胞的细胞质中观察到SGLT1和SGLT2的强染色。在T-2毒素组鸡的肾脏中，SGLT1和SGLT2表达减弱并伴有形态学改变，表明在T-2霉菌毒素中毒期间泌尿系统中的葡萄糖转运减少。
