Ayan A, Aslan Çelik B, Çelik Ö Y, Yılmaz A B, Orunç Kılınç Ö, Oktay Ayan Ö
Department of Genetics, Faculty of Veterinary Medicine, Van Yuzuncu Yil University, Van, Türkiye.
Department of Parasitology, Faculty of Veterinary Medicine, Siirt University, Siirt, Türkiye.
Pol J Vet Sci. 2024 Sep;27(3):389-395. doi: 10.24425/pjvs.2024.151732.
Blastocystis is an intestinal protist commonly found in humans and many different animal species. It is probably the most common enteric parasite with an estimated one billion infections worldwide. The fecal materials for this study were collected from 100 cats and 200 dogs of different ages and sexes in shelters in Van, Turkey. DNA extraction, PCR amplification, and sequence analysis were performed on the fecal samples. A prevalence of 1% (1/100) in cats and 1.5% (3/200) in dogs was detected. The prevalence was higher in both cats and dogs in age groups younger than one year and in females. Sequence analysis revealed Blastocystis sp. ST10 in cats and Blastocystis sp. ST1, ST10, and ST30 in dogs. The sequences obtained were deposited in GenBank. In conclusion, stray cats and dogs may be a source of infection for other cats and dogs, and the detection of zoonotic ST1 in dogs suggests that dogs may be a reservoir for human infection.
芽囊原虫是一种常见于人类和许多不同动物物种的肠道原生生物。它可能是最常见的肠道寄生虫，据估计全球有10亿人感染。本研究的粪便样本取自土耳其凡城收容所中100只不同年龄和性别的猫以及200只不同年龄和性别的狗。对粪便样本进行了DNA提取、PCR扩增和序列分析。在猫中检测到的感染率为1%（1/100），在狗中为1.5%（3/200）。一岁以下年龄组的猫和狗以及雌性的感染率更高。序列分析显示猫中存在芽囊原虫ST10亚型，狗中存在芽囊原虫ST1、ST10和ST30亚型。获得的序列已存入GenBank。总之，流浪猫和狗可能是其他猫和狗的感染源，并且在狗中检测到人畜共患的ST1亚型表明狗可能是人类感染的储存宿主。
