Gao Zhen-Qiu, Gao Yang, Zhuang Hong-Di, Bao Guang-Rong, Liu Jing, Li Jian-Ming, Xue Nian-Yu, Cao Hong-Wei, Liu Shuo
School of Pharmacy, Yancheng Teachers University, Yancheng, Jiangsu Province, China.
College of Life Sciences, Changchun Sci-Tech University, Shuangyang, Jilin Province, China.
Front Vet Sci. 2025 Apr 7;12:1562814. doi: 10.3389/fvets.2025.1562814. eCollection 2025.
sp. is a widespread intestinal protist, that threatens the health of humans and animals. However, epidemiological data on sp. in sika deer are still scarce in China and globally. This study aimed to reveal the infection rate, and subtype distribution of sp. in farmed sika deer across China.
A total of 466 fresh fecal samples were collected from farmed sika deer in northern China. A 600 bp fragment of the SSU rRNA was amplified to detect the presence of sp. in samples.
The results revealed an overall infection rate of sp. at 65.02% (303/466). The highest infection rate was found in Shandong (98%, 49/50), followed by Heilongjiang (95.51%, 85/89), while the lowest infection rate was found in Jilin (36.31%, 61/168). Additionally, within the seasonal group, the infection rate was significantly higher in autumn (69.04%) than in summer (43.06%). In the age group, although the infection rate in young animals (68.38%) was higher than in adults (63.90%), no statistical difference was observed. Ten subtypes were identified from 303 sp.-positive samples, including ST1, ST5, ST10, ST14, ST21, ST23, ST24, ST26, ST30 and ST42. Among these, ST10 was the dominant subtype with an infection rate of 43.23% (131/303), and ST1 and ST5 were zoonotic subtypes. Notably, this study is the first to report the presence of ST42 in sika deer.
These findings suggest that sika deer may be a potential source of human sp. infection. In addition, this study reveals the high infection rate of sp. in farmed sika deer and reports for the first time the presence of ST42 subtype, providing valuable data for the epidemiological study of sp. in sika deer.
[物种名称]是一种广泛存在的肠道原生生物，威胁着人类和动物的健康。然而，在中国乃至全球，关于梅花鹿感染[物种名称]的流行病学数据仍然匮乏。本研究旨在揭示中国养殖梅花鹿中[物种名称]的感染率及亚型分布情况。
从中国北方养殖的梅花鹿中总共采集了466份新鲜粪便样本。扩增小亚基核糖体RNA（SSU rRNA）的一个600 bp片段，以检测样本中[物种名称]的存在情况。
结果显示[物种名称]的总体感染率为65.02%（303/466）。感染率最高的是山东（98%，49/50），其次是黑龙江（95.51%，85/89），而吉林的感染率最低（36.31%，61/168）。此外，在季节分组中，秋季的感染率（69.04%）显著高于夏季（43.06%）。在年龄分组中，虽然幼龄动物的感染率（68.38%）高于成年动物（63.90%），但未观察到统计学差异。从303份[物种名称]阳性样本中鉴定出10种亚型，包括ST1、ST5、ST10、ST14、ST21、ST23、ST24、ST26、ST30和ST42。其中，ST10是优势亚型，感染率为43.23%（131/303），ST1和ST5为人畜共患亚型。值得注意的是，本研究首次报道梅花鹿中存在ST42。
这些发现表明梅花鹿可能是人类感染[物种名称]的潜在来源。此外，本研究揭示了养殖梅花鹿中[物种名称]的高感染率，并首次报道了ST42亚型的存在，为梅花鹿中[物种名称]的流行病学研究提供了有价值的数据。
