Sefid-Sefidehkhan Yasaman, Jouyban Abolghasem, Soleymani Jafar, Khoubnasabjafari Maryam, Jouyban-Gharamaleki Vahid, Rahimpour Elaheh
Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Anesthesiology and Intensive Care, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Mar 15;329:125612. doi: 10.1016/j.saa.2024.125612. Epub 2024 Dec 19.
In this work, we explored the potential of the spot test combined with image analysis using smartphones as a rapid, simple, low-cost, and environmentally friendly method for identifying methadone concentration. Herein, a carbon-gold nanocomposite has been used to generate color variation at different concentrations of methadone. The data obtained from the digital image colorimetric method was compared with those from the UV-Vis spectroscopy as a standard technique. This method was also utilized for extensive optimization and validation procedures. Through image analysis, it can be obtained with the PhotoMetrix smartphone App. and single-variable calibration of collected images. This program computes and processes image histograms from the smartphone camera automatically to determine the concentration of methadone in biological samples. For further analysis, the multivariate calibration technique of PARAFAC can also be used on the images that were taken inside the MATLAB program.
在这项工作中，我们探索了将现场测试与使用智能手机的图像分析相结合，作为一种快速、简单、低成本且环保的美沙酮浓度识别方法的潜力。在此，一种碳金纳米复合材料已被用于在不同浓度的美沙酮下产生颜色变化。将从数字图像比色法获得的数据与作为标准技术的紫外可见光谱法的数据进行了比较。该方法还用于广泛的优化和验证程序。通过图像分析，可以使用PhotoMetrix智能手机应用程序并对收集的图像进行单变量校准来获得结果。该程序会自动计算并处理来自智能手机摄像头的图像直方图，以确定生物样品中美沙酮的浓度。为了进一步分析，PARAFAC多元校准技术也可用于在MATLAB程序中拍摄的图像。
