Radwan Aya Saad, El Hamd Mohamed A, El-Maghrabey Mahmoud, Al-Khateeb Lateefa A, Mahdi Wael A, Alshehri Sultan, Alsaggaf Wejdan T, Saleh Safaa F, Ibrahim Angum M M, Alsehli Bandar R, Magdy Galal
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Horus University-Egypt, New Damietta, Egypt.
Department of Pharmaceutical Chemistry, College of Pharmacy, Shaqra University, Shaqra 11961, Saudi Arabia; Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, South Valley University, Qena 83523, Egypt.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Mar 15;329:125644. doi: 10.1016/j.saa.2024.125644. Epub 2024 Dec 19.
The current study introduces the first micellar-enhanced spectrofluorimetric approach for the estimation of the commonly abused CNS antitussive, dextromethorphan (DXM) in its syrup and biological fluids. A micellar solution of sodium dodecyl sulfate (SDS) containing DXM showed high native fluorescence emission at 305 nm following excitation at 224 nm. Using SDS as a micellar system resulted in about a 2.5-fold increase in the drug's fluorescence intensity and quantum yield as well as the sensitivity of the approach. A thorough investigation was conducted into the experimental factors affecting the studied drug's spectrofluorimetric behavior. Additionally, the quantum yield of DXM was calculated, and it was found to reach up to 22 %. A calibration plot with a straight line was produced across the concentration range of 10.0-200.0 ng/mL. The suggested approach demonstrated excellent sensitivity down to the nanogram level, with 1.80 ng/mL for the detection limit and 5.47 ng/mL for the quantification limit. The drug under study was successfully analyzed in syrup using the designed approach, which yielded low %RSD values (≤0.882) and high %recoveries (99.20-101.00). The efficacy of the suggested fluorimetric technique in detecting DXM in human plasma and urine samples has been demonstrated with excellent recovery (98.12-101.35) and %RSD (≤1.39) values owing to its high sensitivity and selectivity. As DXM is one of the most commonly abused CNS antitussives, the capacity of the proposed method for its analysis in biological fluids can provide further insights for monitoring its potential abuse. The excellent greenness and eco-friendliness of the method were confirmed using GAPI and AGREE metrics, while the BAGI tool assessed its economy, practicality, and applicability. The method was fully validated according to ICH Q2 (R2) guidelines.
本研究介绍了第一种胶束增强荧光光谱法，用于测定糖浆和生物体液中常用的中枢神经系统镇咳药右美沙芬（DXM）。含有DXM的十二烷基硫酸钠（SDS）胶束溶液在224nm激发后，在305nm处显示出高的天然荧光发射。使用SDS作为胶束体系，使药物的荧光强度、量子产率以及该方法的灵敏度提高了约2.5倍。对影响所研究药物荧光光谱行为的实验因素进行了深入研究。此外，计算了DXM的量子产率，发现其高达22%。在10.0 - 200.0 ng/mL的浓度范围内绘制了直线校准曲线。所建议的方法显示出优异的灵敏度，低至纳克水平，检测限为1.80 ng/mL，定量限为5.47 ng/mL。使用所设计的方法成功地分析了糖浆中的研究药物，得到了低的相对标准偏差值（≤0.882）和高的回收率（99.20 - 101.00）。所建议的荧光技术在检测人血浆和尿液样本中的DXM方面的有效性已得到证明，由于其高灵敏度和选择性，回收率优异（98.12 - 101.35），相对标准偏差（≤1.39）。由于DXM是最常用的被滥用中枢神经系统镇咳药之一，所提出的方法在生物体液中分析它的能力可为监测其潜在滥用提供进一步的见解。使用GAPI和AGREE指标证实了该方法具有出色的绿色度和生态友好性，而BAGI工具评估了其经济性、实用性和适用性。该方法根据ICH Q2（R2）指南进行了全面验证。
