He Jiangling, Xu Xingxing, Zhang Yi, Jiang Shangjun, Lin Qingfeng, Zhang Huijuan, Xiong Xiaoxing, Wang Yanying, Deng Hongping, Li Chunya
National R&D Center for Se-Rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-Rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China.
Key Laboratory of Catalysis and Energy Materials Chemistry of Ministry of Education & Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, School of Chemistry and Materials Science, South-Central Minzu University, Wuhan, 430074, China.
Talanta. 2025 May 1;286:127488. doi: 10.1016/j.talanta.2024.127488. Epub 2024 Dec 26.
The mortality rate of tumor is still very high till now. Circulating tumor cells (CTCs) are the major culprit of high cancer mortality. To improve survival rate of cancer patients, real-time monitoring and quantitative detection of CTCs are of indescribable value. However, due to the extremely small content and heterogeneous characteristics of CTCs, to accurately detect CTCs is still a tremendous challenge in clinical trials. Herein, the photoelectrochemical aptasensing and fluorescence imaging were co-joint to detect MCF-7 cells in whole blood via an inertial separation microfluidic chip. A portable inertial microfluidic chip with a height of 150 μm was designed to separate MCF-7 cells from whole blood samples. Niblue-C6-IMC was used to label and image MCF-7 cells through specifically reacting with COX-2 enzyme from the over-expression on the cellular surface. Subsequently, MCF-7 cells were detected with a BiOS nanoflower based photoelectrochemical sensing system. Parameters including the channel height and length, flow rate, focusing position, fluorescence probe concentration, and flow length of the microfluidic chip were optimized. The separation efficiency and purity of MCF-7 cells were 85 % and 80 %, respectively. For 1 mL of blood sample (100 cells mL), 85 cells and 90 cells could be calculated based on the signals from fluorescence imaging and photoelectrochemical response, respectively. Nevertheless, for assaying 1 mL of blood samples containing 50 MCF-7 cells, 40 cells and 39 cells were obtained from fluorescence imaging and photoelectrochemical responses, respectively.
迄今为止，肿瘤的死亡率仍然很高。循环肿瘤细胞（CTCs）是癌症高死亡率的主要元凶。为了提高癌症患者的生存率，对CTCs进行实时监测和定量检测具有不可估量的价值。然而，由于CTCs含量极低且具有异质性，在临床试验中准确检测CTCs仍然是一项巨大的挑战。在此，通过惯性分离微流控芯片将光电化学适体传感和荧光成像联合起来检测全血中的MCF-7细胞。设计了一种高度为150μm的便携式惯性微流控芯片，用于从全血样本中分离MCF-7细胞。利用Niblue-C6-IMC通过与细胞表面过表达的COX-2酶特异性反应来标记和成像MCF-7细胞。随后，使用基于生物纳米花的光电化学传感系统检测MCF-7细胞。对微流控芯片的通道高度和长度、流速、聚焦位置、荧光探针浓度和流动长度等参数进行了优化。MCF-7细胞的分离效率和纯度分别为85%和80%。对于1mL血样（100个细胞/mL），基于荧光成像和光电化学反应的信号分别可计算出85个细胞和90个细胞。然而，对于检测含有50个MCF-7细胞的1mL血样，从荧光成像和光电化学反应中分别获得了40个细胞和39个细胞。
