Vito C C, Baum M J, Bloom C, Fox T O
J Neurosci. 1985 Feb;5(2):268-74. doi: 10.1523/JNEUROSCI.05-02-00268.1985.
Using DNA-cellulose affinity chromatography and either 3H-labeled dihydrotestosterone, testosterone, or estradiol, we qualitatively and quantitatively analyzed the androgen- and estrogen-binding activities present in four regions of male and female ferret brain at prenatal, early and late neonatal, and adult ages. The cytosolic androgen- and estrogen-binding activities in ferret brain at all ages studied were qualitatively similar in both sexes and in all brain regions and exhibited characteristics which resemble those of androgen and estrogen receptors from other species, including rodents and nonhuman primates. A developmental analysis indicated that high levels of both androgen and estrogen receptors were present in the hypothalamus-preoptic area as early as 5 days before birth. A significant, transient decline in concentrations of estrogen receptors (approximately 5-fold) occurred in anterior hypothalamus-preoptic area and mediobasal hypothalamus at 12 days of age in both males and females; this phenomenon has not been observed in any other species studied to date. The observed ontogeny of androgen receptors correlates with the known ability of testosterone, acting over postnatal days 5 to 20, to cause coital masculinization in ferrets, whereas the observed postnatal dip in estradiol receptor concentrations correlates with the inability of estradiol to cause coital masculinization or defeminization of receptive behavior in this species.
利用DNA - 纤维素亲和色谱法以及3H标记的二氢睾酮、睾酮或雌二醇，我们对雄性和雌性雪貂大脑在产前、新生儿早期和晚期以及成年期四个区域中存在的雄激素和雌激素结合活性进行了定性和定量分析。在所研究的所有年龄段，雪貂大脑中的胞质雄激素和雌激素结合活性在性别和所有脑区在定性上都是相似的，并且表现出与包括啮齿动物和非人类灵长类动物在内的其他物种的雄激素和雌激素受体相似的特征。发育分析表明，早在出生前5天，下丘脑 - 视前区就存在高水平的雄激素和雌激素受体。在12日龄时，雄性和雌性雪貂的下丘脑前视前区和下丘脑中间基底部的雌激素受体浓度均出现显著的、短暂的下降（约5倍）；这种现象在迄今为止研究的任何其他物种中均未观察到。观察到的雄激素受体个体发生与已知的睾酮在出生后第5至20天发挥作用导致雪貂交配行为雄性化的能力相关，而观察到的出生后雌二醇受体浓度下降与雌二醇无法导致该物种交配行为的雄性化或去雌性化相关。
